Potential for biodegradation of polychlorinated biphenyls (PCBs) by Sinorhizobium meliloti

Chen Tu; Ying Teng; Yongming Luo; Xiuhua Li; Xianghui Sun; Zhengao Li; Wuxing Liu; Peter Christie

doi:10.1016/j.jhazmat.2010.12.008

Potential for biodegradation of polychlorinated biphenyls (PCBs) by Sinorhizobium meliloti

Chen Tu, Ying Teng, Yongming Luo^*, Xiuhua Li, Xianghui Sun, Zhengao Li, Wuxing Liu, Peter Christie

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

Resting cell assay and soil microcosms were set up to investigate the biodegradation capability and metabolic intermediate of polychlorinated biphenyls (PCBs) by a rhizobial strain Sinorhizobium meliloti. Biodegradation was observed immediately after 2,4,4′-TCB was supplied as a sole source of carbon and energy in liquid cultures. After 6 days, the percent biodegradation of 2,4,4′-TCB was 77.4% compared with the control. The main intermediate was identified as 2-hydroxy-6-oxo-6-phenylhex-2,4-dienoic acid (HOPDA) for 2,4,4′-TCB as determined by gas chromatography–mass spectrometry (GC–MS). Inoculation with S. meliloti greatly enhanced the degradation of target PCB mixtures in the soil. Moreover, soil culturable bacteria, fungi and biphenyl degrading bacteria counts showed significant increase after inoculation of S. meliloti. This study suggests that S. meliloti is promising in PCB bioremediation.

Original language	English
Pages (from-to)	1438-1444
Journal	Journal of Hazardous Materials
Volume	186
Issue number	2-3
Early online date	10 Dec 2010
DOIs	https://doi.org/10.1016/j.jhazmat.2010.12.008
Publication status	Published - 28 Feb 2011
Externally published	Yes

ASJC Scopus subject areas

Health, Toxicology and Mutagenesis
Pollution
Waste Management and Disposal
Environmental Chemistry
Environmental Engineering

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@article{374aef8ec4aa415787a5c4cd74872d4e,

title = "Potential for biodegradation of polychlorinated biphenyls (PCBs) by Sinorhizobium meliloti",

abstract = "Resting cell assay and soil microcosms were set up to investigate the biodegradation capability and metabolic intermediate of polychlorinated biphenyls (PCBs) by a rhizobial strain Sinorhizobium meliloti. Biodegradation was observed immediately after 2,4,4′-TCB was supplied as a sole source of carbon and energy in liquid cultures. After 6 days, the percent biodegradation of 2,4,4′-TCB was 77.4% compared with the control. The main intermediate was identified as 2-hydroxy-6-oxo-6-phenylhex-2,4-dienoic acid (HOPDA) for 2,4,4′-TCB as determined by gas chromatography–mass spectrometry (GC–MS). Inoculation with S. meliloti greatly enhanced the degradation of target PCB mixtures in the soil. Moreover, soil culturable bacteria, fungi and biphenyl degrading bacteria counts showed significant increase after inoculation of S. meliloti. This study suggests that S. meliloti is promising in PCB bioremediation.",

author = "Chen Tu and Ying Teng and Yongming Luo and Xiuhua Li and Xianghui Sun and Zhengao Li and Wuxing Liu and Peter Christie",

year = "2011",

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doi = "10.1016/j.jhazmat.2010.12.008",

language = "English",

volume = "186",

pages = "1438--1444",

journal = "Journal of Hazardous Materials",

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T1 - Potential for biodegradation of polychlorinated biphenyls (PCBs) by Sinorhizobium meliloti

AU - Tu, Chen

AU - Teng, Ying

AU - Luo, Yongming

AU - Li, Xiuhua

AU - Sun, Xianghui

AU - Li, Zhengao

AU - Liu, Wuxing

AU - Christie, Peter

PY - 2011/2/28

Y1 - 2011/2/28

N2 - Resting cell assay and soil microcosms were set up to investigate the biodegradation capability and metabolic intermediate of polychlorinated biphenyls (PCBs) by a rhizobial strain Sinorhizobium meliloti. Biodegradation was observed immediately after 2,4,4′-TCB was supplied as a sole source of carbon and energy in liquid cultures. After 6 days, the percent biodegradation of 2,4,4′-TCB was 77.4% compared with the control. The main intermediate was identified as 2-hydroxy-6-oxo-6-phenylhex-2,4-dienoic acid (HOPDA) for 2,4,4′-TCB as determined by gas chromatography–mass spectrometry (GC–MS). Inoculation with S. meliloti greatly enhanced the degradation of target PCB mixtures in the soil. Moreover, soil culturable bacteria, fungi and biphenyl degrading bacteria counts showed significant increase after inoculation of S. meliloti. This study suggests that S. meliloti is promising in PCB bioremediation.

AB - Resting cell assay and soil microcosms were set up to investigate the biodegradation capability and metabolic intermediate of polychlorinated biphenyls (PCBs) by a rhizobial strain Sinorhizobium meliloti. Biodegradation was observed immediately after 2,4,4′-TCB was supplied as a sole source of carbon and energy in liquid cultures. After 6 days, the percent biodegradation of 2,4,4′-TCB was 77.4% compared with the control. The main intermediate was identified as 2-hydroxy-6-oxo-6-phenylhex-2,4-dienoic acid (HOPDA) for 2,4,4′-TCB as determined by gas chromatography–mass spectrometry (GC–MS). Inoculation with S. meliloti greatly enhanced the degradation of target PCB mixtures in the soil. Moreover, soil culturable bacteria, fungi and biphenyl degrading bacteria counts showed significant increase after inoculation of S. meliloti. This study suggests that S. meliloti is promising in PCB bioremediation.

U2 - 10.1016/j.jhazmat.2010.12.008

DO - 10.1016/j.jhazmat.2010.12.008

M3 - Article

SN - 0304-3894

VL - 186

SP - 1438

EP - 1444

JO - Journal of Hazardous Materials

JF - Journal of Hazardous Materials

IS - 2-3

ER -

Potential for biodegradation of polychlorinated biphenyls (PCBs) by Sinorhizobium meliloti

Abstract

ASJC Scopus subject areas

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