Preceding exercise and postprandial hypertriglyceridemia: effects on lymphocyte cell DNA damage and vascular inflammation

Malcolm Brown, Conor M. McClean, Gareth W. Davison, John C. W. Brown, Marie H. Murphy

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Abstract

Background: Exercise has proved effective in attenuating the unfavourable response normally associated with postprandial hypertriglyceridemia (PHTG) and accompanying oxidative stress. Yet, the acute effects of prior exercise and PHTG on DNA damage remains unknown. The purpose of this study was to examine if walking alters PHTGinduced oxidative damage and the interrelated inflammatory mechanisms.Methods: Twelve apparently healthy, recreationally active, male participants (22.4 ± 4.1 years; 179.2 ± 6 cm; 84.2 ± 14.7 kg; 51.3 ± 8.6 ml·kg− 1·min− 1) completed a randomised, crossover study consisting of two trials: (1) a high-fat mealalone (resting control) or (2) a high-fat meal immediately following 1 h of moderate exercise (65% maximal heartrate). Venous blood samples were collected at baseline, immediately post-exercise or rest, as well as at 2, 4 and 6 hpost-meal. Biomarkers of oxidative damage (DNA single-strand breaks, lipid peroxidation and free radicalmetabolism) and inflammation were determined using conventional biochemistry techniques.Results: DNA damage, lipid peroxidation, free radical metabolism and triglycerides increased postprandially (main effect for time, p < 0.05), regardless of completing 1 h of preceding moderate intensity exercise. Plasma antioxidants(α-tocopherol and γ-tocopherol) also mobilised in response to the high-fat meal (main effect for time, p < 0.05), butno changes were detected for retinol-binding protein-4.Conclusion: The ingestion of a high fat meal induces postprandial oxidative stress, inflammation and a rise in DNA damage that remains unaltered by one hour of preceding exercise.Keywords: Exercise, Postprandial hypertriglyceridemia, Oxidative stress, DNA, Inflammation
Original languageEnglish
Article number125
JournalLipids in health and disease
Volume18
Publication statusPublished - 29 May 2019

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