Preparation of planar retinal specimens: verification by histology, mRNA profiling, and proteome analysis

Gareth J McKay, Lucinda Campbell, Mike Oliver, Simon Brockbank, David A C Simpson, William J Curry

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Elucidation of the transcriptome and proteome of the normal retina will be difficult since it is comprised of at least 55 different cell types. However the characteristic layered cellular anatomy of the retina makes it amenable to planar sectioning, enabling the generation of enriched retinal cell populations. The aim of this study was to validate a reproducible method for preparing enriched retinal layers from porcine retina.
Original languageEnglish
Pages (from-to)240-7
Number of pages8
JournalMolecular Vision
Volume10
Publication statusPublished - 29 Mar 2004

Keywords

  • Anatomy, Cross-Sectional
  • Animals
  • Chromatography, Liquid
  • Cryoultramicrotomy
  • Eye Proteins
  • Gene Expression
  • Gene Expression Profiling
  • Mass Spectrometry
  • Oligonucleotide Array Sequence Analysis
  • Peptide Fragments
  • Proteome
  • RNA, Messenger
  • Reproducibility of Results
  • Retina
  • Reverse Transcriptase Polymerase Chain Reaction
  • Specimen Handling
  • Swine

Fingerprint Dive into the research topics of 'Preparation of planar retinal specimens: verification by histology, mRNA profiling, and proteome analysis'. Together they form a unique fingerprint.

Cite this