Presence and activity of HPV in primary lung cancer

Talita Helena Araujo de Oliveira; Carolina Medeiros  do Amaral; Bianca  de França São Marcos; Kamylla Conceição  Gomes Nascimento; Ana Carine  de Miranda Rios; Dafne Carolina Alves Quixabeira; Maria Tereza Cartaxo Muniz; Jacinto  da Costa Silva Neto; Antonio Carlos de Freitas

doi:10.1007/s00432-018-2748-8

Presence and activity of HPV in primary lung cancer

Talita Helena Araujo de Oliveira
, Carolina Medeiros do Amaral
, Bianca de França São Marcos
, Kamylla Conceição Gomes Nascimento
, Ana Carine de Miranda Rios
, Dafne Carolina Alves Quixabeira
, Maria Tereza Cartaxo Muniz
, Jacinto da Costa Silva Neto
, Antonio Carlos de Freitas^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

Purpose
Evaluate the presence of human papillomavirus (HPV) in biopsies of Brazilian patients with lung cancer and also the expression of the E6 and E7 oncoproteins. HPV is widely known as an important condition for cervical cancer although evidence today shows it is associated with several other types of cancer and may also be involved in lung cancer development. However, there are some divergences regarding the presence and activity of HPV in lung carcinogenesis.

Methods
The detection of HPV was performed by PCR, followed by genotype and immunohistochemical evaluation of E6 and E7 HPV type specific, from 63 patients.

Results
HPV was found to be present in 33 of the 63 samples, and types 16 and 18 were detected with frequencies of 81% (27/33) and 19% (6/33), respectively. About the presence of the virus in different histological types of tumors, HPV was detected in squamous cell carcinoma (39.39%), followed by adenocarcinoma (33.33%) and small cell carcinoma (18.18%) and large cell carcinoma (9.1%). The presence of the E6 (antibody anti-HPV 16 and anti-HPV 18) and E7 (antibody anti-HPV 16 and anti-HPV 18) oncoproteins was detected by immunohistochemical stain technique in 28/33 samples and 25/33 samples, respectively.

Conclusions
Our results suggest that the lung tumor patients have high prevalence of HPV and the virus is not only present but also active in tumor cells. Therefore, the HPV is probably playing a role in lung carcinogenesis.

Original language	English
Pages (from-to)	2367–2376
Journal	Journal of Cancer Research and Clinical Oncology
Volume	144
Issue number	12
Early online date	17 Sept 2018
DOIs	https://doi.org/10.1007/s00432-018-2748-8
Publication status	Published - Dec 2018
Externally published	Yes

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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de Oliveira, T. H. A., do Amaral, C. M., de França São Marcos, B., Gomes Nascimento, K. C., de Miranda Rios, A. C., Quixabeira, D. C. A., Muniz, M. T. C., da Costa Silva Neto, J., & de Freitas, A. C. (2018). Presence and activity of HPV in primary lung cancer. Journal of Cancer Research and Clinical Oncology, 144(12), 2367–2376. https://doi.org/10.1007/s00432-018-2748-8

@article{ac5fb61163f7478aa6d6d667c688d25f,

title = "Presence and activity of HPV in primary lung cancer",

abstract = "Purpose Evaluate the presence of human papillomavirus (HPV) in biopsies of Brazilian patients with lung cancer and also the expression of the E6 and E7 oncoproteins. HPV is widely known as an important condition for cervical cancer although evidence today shows it is associated with several other types of cancer and may also be involved in lung cancer development. However, there are some divergences regarding the presence and activity of HPV in lung carcinogenesis. Methods The detection of HPV was performed by PCR, followed by genotype and immunohistochemical evaluation of E6 and E7 HPV type specific, from 63 patients. Results HPV was found to be present in 33 of the 63 samples, and types 16 and 18 were detected with frequencies of 81\% (27/33) and 19\% (6/33), respectively. About the presence of the virus in different histological types of tumors, HPV was detected in squamous cell carcinoma (39.39\%), followed by adenocarcinoma (33.33\%) and small cell carcinoma (18.18\%) and large cell carcinoma (9.1\%). The presence of the E6 (antibody anti-HPV 16 and anti-HPV 18) and E7 (antibody anti-HPV 16 and anti-HPV 18) oncoproteins was detected by immunohistochemical stain technique in 28/33 samples and 25/33 samples, respectively. Conclusions Our results suggest that the lung tumor patients have high prevalence of HPV and the virus is not only present but also active in tumor cells. Therefore, the HPV is probably playing a role in lung carcinogenesis.",

author = "\{de Oliveira\}, \{Talita Helena Araujo\} and \{do Amaral\}, \{Carolina Medeiros\} and \{de Fran{\c c}a S{\~a}o Marcos\}, Bianca and \{Gomes Nascimento\}, \{Kamylla Concei{\c c}{\~a}o\} and \{de Miranda Rios\}, \{Ana Carine\} and Quixabeira, \{Dafne Carolina Alves\} and Muniz, \{Maria Tereza Cartaxo\} and \{da Costa Silva Neto\}, Jacinto and \{de Freitas\}, \{Antonio Carlos\}",

year = "2018",

month = dec,

doi = "10.1007/s00432-018-2748-8",

language = "English",

volume = "144",

pages = "2367–2376",

journal = "Journal of Cancer Research and Clinical Oncology",

publisher = "Springer Science and Business Media Deutschland GmbH",

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T1 - Presence and activity of HPV in primary lung cancer

AU - de Oliveira, Talita Helena Araujo

AU - do Amaral, Carolina Medeiros

AU - de França São Marcos, Bianca

AU - Gomes Nascimento, Kamylla Conceição

AU - de Miranda Rios, Ana Carine

AU - Quixabeira, Dafne Carolina Alves

AU - Muniz, Maria Tereza Cartaxo

AU - da Costa Silva Neto, Jacinto

AU - de Freitas, Antonio Carlos

PY - 2018/12

Y1 - 2018/12

N2 - Purpose Evaluate the presence of human papillomavirus (HPV) in biopsies of Brazilian patients with lung cancer and also the expression of the E6 and E7 oncoproteins. HPV is widely known as an important condition for cervical cancer although evidence today shows it is associated with several other types of cancer and may also be involved in lung cancer development. However, there are some divergences regarding the presence and activity of HPV in lung carcinogenesis. Methods The detection of HPV was performed by PCR, followed by genotype and immunohistochemical evaluation of E6 and E7 HPV type specific, from 63 patients. Results HPV was found to be present in 33 of the 63 samples, and types 16 and 18 were detected with frequencies of 81% (27/33) and 19% (6/33), respectively. About the presence of the virus in different histological types of tumors, HPV was detected in squamous cell carcinoma (39.39%), followed by adenocarcinoma (33.33%) and small cell carcinoma (18.18%) and large cell carcinoma (9.1%). The presence of the E6 (antibody anti-HPV 16 and anti-HPV 18) and E7 (antibody anti-HPV 16 and anti-HPV 18) oncoproteins was detected by immunohistochemical stain technique in 28/33 samples and 25/33 samples, respectively. Conclusions Our results suggest that the lung tumor patients have high prevalence of HPV and the virus is not only present but also active in tumor cells. Therefore, the HPV is probably playing a role in lung carcinogenesis.

AB - Purpose Evaluate the presence of human papillomavirus (HPV) in biopsies of Brazilian patients with lung cancer and also the expression of the E6 and E7 oncoproteins. HPV is widely known as an important condition for cervical cancer although evidence today shows it is associated with several other types of cancer and may also be involved in lung cancer development. However, there are some divergences regarding the presence and activity of HPV in lung carcinogenesis. Methods The detection of HPV was performed by PCR, followed by genotype and immunohistochemical evaluation of E6 and E7 HPV type specific, from 63 patients. Results HPV was found to be present in 33 of the 63 samples, and types 16 and 18 were detected with frequencies of 81% (27/33) and 19% (6/33), respectively. About the presence of the virus in different histological types of tumors, HPV was detected in squamous cell carcinoma (39.39%), followed by adenocarcinoma (33.33%) and small cell carcinoma (18.18%) and large cell carcinoma (9.1%). The presence of the E6 (antibody anti-HPV 16 and anti-HPV 18) and E7 (antibody anti-HPV 16 and anti-HPV 18) oncoproteins was detected by immunohistochemical stain technique in 28/33 samples and 25/33 samples, respectively. Conclusions Our results suggest that the lung tumor patients have high prevalence of HPV and the virus is not only present but also active in tumor cells. Therefore, the HPV is probably playing a role in lung carcinogenesis.

U2 - 10.1007/s00432-018-2748-8

DO - 10.1007/s00432-018-2748-8

M3 - Article

C2 - 30225539

VL - 144

SP - 2367

EP - 2376

JO - Journal of Cancer Research and Clinical Oncology

JF - Journal of Cancer Research and Clinical Oncology

IS - 12

ER -

Presence and activity of HPV in primary lung cancer

Abstract

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