Prevalence of the factor VR506Q mutation in two Irish control populations: use of a novel nested polymerase chain reaction approach

W J Livingstone, B White, L Mynett-Johnson, M Lawler, P Mayne, O P Smith, Mark Lawler

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The prevalence of factor V (FV) Leiden among normal populations has primarily been determined using blood donors. This control group is carefully selected and therefore may not accurately reflect the true prevalence within the population. We assessed the prevalence of FV Leiden within the Irish population using Guthrie card samples randomly selected from all newborns. We compared this result with the prevalence of FV Leiden within blood donors. A novel nested polymerase chain reaction (PCR) method for FV Leiden was developed for analysis of the Guthrie card samples. There was no significant difference between the allele frequency within the Guthrie card samples and blood donors (2.07% vs. 2.35%, P = 0.66)

Original languageEnglish
Pages (from-to)559-61
Number of pages3
JournalBritish Journal of Haematology
Volume111
Issue number2
Publication statusPublished - Nov 2000

Keywords

  • Adult
  • Aged
  • Blood Donors
  • Factor V
  • Female
  • Gene Frequency
  • Heterozygote
  • Homozygote
  • Humans
  • Infant, Newborn
  • Ireland
  • Male
  • Middle Aged
  • Polymerase Chain Reaction
  • Prevalence

Fingerprint Dive into the research topics of 'Prevalence of the factor VR506Q mutation in two Irish control populations: use of a novel nested polymerase chain reaction approach'. Together they form a unique fingerprint.

Cite this