Production, characterisation and applications of monoclonal antibodies to two novel porcine bocaviruses from swine in Northern Ireland

I. McNair; F. McNeilly; C. Duffy; J. McKillen; M. McMenamy; M. Welsh; Gordon Allan

doi:10.1007/s00705-011-1107-8

Production, characterisation and applications of monoclonal antibodies to two novel porcine bocaviruses from swine in Northern Ireland

I. McNair, F. McNeilly, C. Duffy, J. McKillen, M. McMenamy, M. Welsh, Gordon Allan

School of Biological Sciences

Research output: Contribution to journal › Article

5 Citations (Scopus)

Abstract

The production, preliminary characterisation and applications of monoclonal antibodies (mAbs) against two novel swine bocaviruses isolated in cell culture from swine in Northern Ireland are described. Of the 17 stable final clones produced, four were characterised. All were of the IgG2a isotype and showed no cross-reactivity with either bocavirus strain. Partial neutralisation was observed with PBoV4 mAbs and homologous virus. The two mAbs selected for use in antigen-detecting ELISAs were successful in highlighting those fractions containing infectious virus within sucrose gradients. This is the first report of the production of specific reagents that will prove useful in the study of the biology of these viruses and swine bocavirus-associated diseases.

Original language	English
Pages (from-to)	2157-2162
Number of pages	6
Journal	Archives of Virology
Volume	156
Issue number	12
DOIs	https://doi.org/10.1007/s00705-011-1107-8
Publication status	Published - Dec 2011

ASJC Scopus subject areas

Virology

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title = "Production, characterisation and applications of monoclonal antibodies to two novel porcine bocaviruses from swine in Northern Ireland",

abstract = "The production, preliminary characterisation and applications of monoclonal antibodies (mAbs) against two novel swine bocaviruses isolated in cell culture from swine in Northern Ireland are described. Of the 17 stable final clones produced, four were characterised. All were of the IgG2a isotype and showed no cross-reactivity with either bocavirus strain. Partial neutralisation was observed with PBoV4 mAbs and homologous virus. The two mAbs selected for use in antigen-detecting ELISAs were successful in highlighting those fractions containing infectious virus within sucrose gradients. This is the first report of the production of specific reagents that will prove useful in the study of the biology of these viruses and swine bocavirus-associated diseases.",

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AU - McNair, I.

AU - McNeilly, F.

AU - Duffy, C.

AU - McKillen, J.

AU - McMenamy, M.

AU - Welsh, M.

AU - Allan, Gordon

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AB - The production, preliminary characterisation and applications of monoclonal antibodies (mAbs) against two novel swine bocaviruses isolated in cell culture from swine in Northern Ireland are described. Of the 17 stable final clones produced, four were characterised. All were of the IgG2a isotype and showed no cross-reactivity with either bocavirus strain. Partial neutralisation was observed with PBoV4 mAbs and homologous virus. The two mAbs selected for use in antigen-detecting ELISAs were successful in highlighting those fractions containing infectious virus within sucrose gradients. This is the first report of the production of specific reagents that will prove useful in the study of the biology of these viruses and swine bocavirus-associated diseases.

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JF - Archives of Virology

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