Production, characterisation and applications of monoclonal antibodies to porcine circovirus 2

F. McNeilly, I. McNair, D. P. Mackie, B. M. Meehan, S. Kennedy, D. Moffett, J. Ellis, S. Krakowka, G. M. Allan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

103 Citations (Scopus)

Abstract

The production, preliminary characterisation and applications of monoclonal antibodies (mabs) against six porcine circovirus 2 isolates are described. A total of 14 stable hybridomas were produced, of which 7 were characterised. All of the mabs characterised were of IgG isotype. All the mabs tested reacted by IIF with acetone-fixed cell cultures infected with PCV2 isolates from Canada, France, Spain, Denmark, USA and UK. No cross-reactivity with a porcine circovirus 1 field isolate was demonstrated using the panel of mabs tested. In addition, one of the seven mabs tested demonstrated neutralising activity against PCV2 isolates from Canada and France. The use of selected PCV2-specific mabs for the development of virus detection methodologies is described.

Original languageEnglish
Pages (from-to)909-922
Number of pages14
JournalArchives of Virology
Volume146
Issue number5
DOIs
Publication statusPublished - 2001
Externally publishedYes

Bibliographical note

Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.

ASJC Scopus subject areas

  • Virology

Fingerprint

Dive into the research topics of 'Production, characterisation and applications of monoclonal antibodies to porcine circovirus 2'. Together they form a unique fingerprint.

Cite this