Production of 1,3-Propanediol from Pure and Crude Glycerol Using Immobilised Clostridium Butyricum

Igor Dolejš, Monika Líšková, Vladimír Krasňan, Kristína Markošová, Michal Rosenberg, Fabio Lorenzini, Andrew C. Marr, Martin Rebros

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Abstract

The present study describes the production of the value-added chemical 1,3-propanediol (1,3-PD) from crude glycerol, a waste by-product formed during biodiesel production. The efficiency, robustness, and stability of the process were improved by immobilization of the anaerobic bacterium Clostridium butyricum into a polyvinyl alcohol (PVA) hydrogel. The highest average productivity, 6.8 ± 0.2 g/(L·h), was achieved in 10 consecutive, repeated batch fermentations, with an initial concentration of pure glycerol 45.5 ± 0.7 g/L, after 2.5 hours. The highest final concentration and yield of 1,3-PD, 28.3 ± 0.6 g/L, and 0.42 ± 0.01 g/g, respectively, were achieved in eleven repeated batch fermentations, after increasing the initial pure glycerol concentration to 70.4 ± 1.9 g/L. Two different types of crude glycerol, produced from used cooking oil (UCO) and rapeseed oil (RO), were tested in repeated batch fermentations, with an average productivity achieved of 2.3 ± 0.1 and 3.5 ± 0.3 g/(L·h), respectively. The highest final concentration and yield of 1,3-PD, 12.6 ± 0.9 g/L, and 0.35 ± 0.02 g/g, respectively, were observed in fifteen repeated batch fermentations with RO crude glycerol. An excellent stability of the immobilized anaerobic bacteria and increase of productivity in fermentation of crude glycerol was demonstrated.
Original languageEnglish
Article number317
Number of pages13
JournalCatalysts
Volume9
Issue number4
DOIs
Publication statusPublished - 31 Mar 2019

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Dolejš, I., Líšková, M., Krasňan, V., Markošová, K., Rosenberg, M., Lorenzini, F., ... Rebros, M. (2019). Production of 1,3-Propanediol from Pure and Crude Glycerol Using Immobilised Clostridium Butyricum. Catalysts, 9(4), [317]. https://doi.org/10.3390/catal9040317