Production of a monoclonal antibody and its application in an optical biosensor based assay for the quantitative measurement of Pantothenic Acid (Vitamin B5) in foodstuffs

Simon A. Haughey, Christopher T. Elliott, Michalina Oplatowska, Linda D. Stewart, Caroline Frizzell, Lisa Connolly

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Pantothenicacid (PA), vitamin B5, is an essential B vitamin that may be fortified in food and as such requires robust and accurate methods of detection to meet compliance legislation. This study reports the production and characterisation of the first monoclonalantibody (MAb) specific for PA and the subsequent development of a surface plasmon resonance (SPR) biosensorassay for the quantification of PA. The developed assay was compared with an SPR based commercial kit which utilised a polyclonal antibody (PAb). Foodstuffs, including cereals (n = 43), infant formulas and baby food (n = 10) and fruit juices (n = 48) were analysed by both the MAb and PAb biosensorassays and comparison plots showed good correlation (R2 0.77–0.99). The results indicate that the MAb basedbiosensorassay is suitable for the measurement of PA in foodstuffs and has the added advantage of facilitating a constant, long term supply of identical antibody. Preliminary matrix studies suggest the MAb basedassay is an excellent candidate for further validation studies and routine quality assurance based analysis.
Original languageEnglish
Pages (from-to)540-545
Number of pages5
JournalFood Chemistry
Volume134
Issue number1
Early online date25 Feb 2012
DOIs
Publication statusPublished - Sep 2012

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

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