Prostaglandin E2 as a regulator of germ cells during ovarian development

Rosemary A L Bayne, Sharon L Eddie, Craig S Collins, Andrew J Childs, Henry N Jabbour, Richard A Anderson

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

CONTEXT: The formation of primordial follicles occurs during fetal life yet is critical to the determination of adult female fertility. Prior to this stage, germ cells proliferate, enter meiosis, and associate with somatic cells. Growth and survival factors implicated in these processes include activin A (INHBA), the neurotrophins BDNF and NT4 (NTF5), and MCL1. The prostaglandins have pleiotrophic roles in reproduction, notably in ovulation and implantation, but there are no data regarding roles for prostaglandins in human fetal ovarian development.

OBJECTIVE: The aim of the study was to investigate a possible role for prostaglandin (PG) E(2) in human fetal ovary development.

DESIGN: In vitro analysis of ovarian development between 8 and 20 wk gestation was performed.

MAIN OUTCOME MEASURE(S): The expression patterns of PG synthesis enzymes and the PGE(2) receptors EP2 and EP4 in the ovary were assessed, and downstream effects of PGE(2) on gene expression were analyzed.

RESULTS: Ovarian germ cells express the PG synthetic enzymes COX2 and PTGES as well as the EP2 and EP4 receptors, whereas COX1 is expressed by ovarian somatic cells. Treatment in vitro with PGE(2) increased the expression of BDNF mRNA 1.7 +/- 0.16-fold (P = 0.004); INHBA mRNA, 2.1 +/- 0.51-fold (P = 0.04); and MCL1 mRNA, 1.15 +/- 0.06-fold (P = 0.04), but not that of OCT4, DAZL, VASA, NTF5, or SMAD3.

CONCLUSIONS: These data indicate novel roles for PGE(2) in the regulation of germ cell development in the human ovary and show that these effects may be mediated by the regulation of factors including BDNF, activin A, and MCL1.

Original languageEnglish
Pages (from-to)4053-60
Number of pages8
JournalThe Journal of clinical endocrinology and metabolism
Volume94
Issue number10
DOIs
Publication statusPublished - Oct 2009

Fingerprint

Prostaglandins E
Dinoprostone
Germ Cells
Brain-Derived Neurotrophic Factor
Cells
Prostaglandins
Ovary
Fetal Development
Messenger RNA
Prostaglandin E Receptors
Synthetic Prostaglandins
Nerve Growth Factors
Meiosis
Human Development
Enzymes
Ovulation
Reproduction
Fertility
Intercellular Signaling Peptides and Proteins
Gene expression

Keywords

  • Activins
  • Adult
  • Brain-Derived Neurotrophic Factor
  • Dinoprostone
  • Female
  • Fetus
  • Gene Expression Regulation, Developmental
  • Humans
  • Immunohistochemistry
  • Intramolecular Oxidoreductases
  • Myeloid Cell Leukemia Sequence 1 Protein
  • Oocytes
  • Ovary
  • Pregnancy
  • Proto-Oncogene Proteins c-bcl-2
  • Receptors, Prostaglandin E
  • Receptors, Prostaglandin E, EP2 Subtype
  • Receptors, Prostaglandin E, EP4 Subtype
  • Reverse Transcriptase Polymerase Chain Reaction

Cite this

Bayne, Rosemary A L ; Eddie, Sharon L ; Collins, Craig S ; Childs, Andrew J ; Jabbour, Henry N ; Anderson, Richard A. / Prostaglandin E2 as a regulator of germ cells during ovarian development. In: The Journal of clinical endocrinology and metabolism. 2009 ; Vol. 94, No. 10. pp. 4053-60.
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Prostaglandin E2 as a regulator of germ cells during ovarian development. / Bayne, Rosemary A L; Eddie, Sharon L; Collins, Craig S; Childs, Andrew J; Jabbour, Henry N; Anderson, Richard A.

In: The Journal of clinical endocrinology and metabolism, Vol. 94, No. 10, 10.2009, p. 4053-60.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Prostaglandin E2 as a regulator of germ cells during ovarian development

AU - Bayne, Rosemary A L

AU - Eddie, Sharon L

AU - Collins, Craig S

AU - Childs, Andrew J

AU - Jabbour, Henry N

AU - Anderson, Richard A

PY - 2009/10

Y1 - 2009/10

N2 - CONTEXT: The formation of primordial follicles occurs during fetal life yet is critical to the determination of adult female fertility. Prior to this stage, germ cells proliferate, enter meiosis, and associate with somatic cells. Growth and survival factors implicated in these processes include activin A (INHBA), the neurotrophins BDNF and NT4 (NTF5), and MCL1. The prostaglandins have pleiotrophic roles in reproduction, notably in ovulation and implantation, but there are no data regarding roles for prostaglandins in human fetal ovarian development.OBJECTIVE: The aim of the study was to investigate a possible role for prostaglandin (PG) E(2) in human fetal ovary development.DESIGN: In vitro analysis of ovarian development between 8 and 20 wk gestation was performed.MAIN OUTCOME MEASURE(S): The expression patterns of PG synthesis enzymes and the PGE(2) receptors EP2 and EP4 in the ovary were assessed, and downstream effects of PGE(2) on gene expression were analyzed.RESULTS: Ovarian germ cells express the PG synthetic enzymes COX2 and PTGES as well as the EP2 and EP4 receptors, whereas COX1 is expressed by ovarian somatic cells. Treatment in vitro with PGE(2) increased the expression of BDNF mRNA 1.7 +/- 0.16-fold (P = 0.004); INHBA mRNA, 2.1 +/- 0.51-fold (P = 0.04); and MCL1 mRNA, 1.15 +/- 0.06-fold (P = 0.04), but not that of OCT4, DAZL, VASA, NTF5, or SMAD3.CONCLUSIONS: These data indicate novel roles for PGE(2) in the regulation of germ cell development in the human ovary and show that these effects may be mediated by the regulation of factors including BDNF, activin A, and MCL1.

AB - CONTEXT: The formation of primordial follicles occurs during fetal life yet is critical to the determination of adult female fertility. Prior to this stage, germ cells proliferate, enter meiosis, and associate with somatic cells. Growth and survival factors implicated in these processes include activin A (INHBA), the neurotrophins BDNF and NT4 (NTF5), and MCL1. The prostaglandins have pleiotrophic roles in reproduction, notably in ovulation and implantation, but there are no data regarding roles for prostaglandins in human fetal ovarian development.OBJECTIVE: The aim of the study was to investigate a possible role for prostaglandin (PG) E(2) in human fetal ovary development.DESIGN: In vitro analysis of ovarian development between 8 and 20 wk gestation was performed.MAIN OUTCOME MEASURE(S): The expression patterns of PG synthesis enzymes and the PGE(2) receptors EP2 and EP4 in the ovary were assessed, and downstream effects of PGE(2) on gene expression were analyzed.RESULTS: Ovarian germ cells express the PG synthetic enzymes COX2 and PTGES as well as the EP2 and EP4 receptors, whereas COX1 is expressed by ovarian somatic cells. Treatment in vitro with PGE(2) increased the expression of BDNF mRNA 1.7 +/- 0.16-fold (P = 0.004); INHBA mRNA, 2.1 +/- 0.51-fold (P = 0.04); and MCL1 mRNA, 1.15 +/- 0.06-fold (P = 0.04), but not that of OCT4, DAZL, VASA, NTF5, or SMAD3.CONCLUSIONS: These data indicate novel roles for PGE(2) in the regulation of germ cell development in the human ovary and show that these effects may be mediated by the regulation of factors including BDNF, activin A, and MCL1.

KW - Activins

KW - Adult

KW - Brain-Derived Neurotrophic Factor

KW - Dinoprostone

KW - Female

KW - Fetus

KW - Gene Expression Regulation, Developmental

KW - Humans

KW - Immunohistochemistry

KW - Intramolecular Oxidoreductases

KW - Myeloid Cell Leukemia Sequence 1 Protein

KW - Oocytes

KW - Ovary

KW - Pregnancy

KW - Proto-Oncogene Proteins c-bcl-2

KW - Receptors, Prostaglandin E

KW - Receptors, Prostaglandin E, EP2 Subtype

KW - Receptors, Prostaglandin E, EP4 Subtype

KW - Reverse Transcriptase Polymerase Chain Reaction

U2 - 10.1210/jc.2009-0755

DO - 10.1210/jc.2009-0755

M3 - Article

C2 - 19602557

VL - 94

SP - 4053

EP - 4060

JO - The Journal of Clinical Endocrinology & Metabolism

JF - The Journal of Clinical Endocrinology & Metabolism

SN - 0021-972X

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ER -