Protein destabilisation by ruthenium(II) tris-bipyridine based protein-surface mimetics

Andrew J. Wilson; James R. Ault; Maria H. Filby; Hazel I. A. Phillips; Alison E. Ashcroft; Nicholas C. Fletcher

doi:10.1039/c3ob26251k

Protein destabilisation by ruthenium(II) tris-bipyridine based protein-surface mimetics

Andrew J. Wilson, James R. Ault, Maria H. Filby, Hazel I. A. Phillips, Alison E. Ashcroft, Nicholas C. Fletcher

School of Chemistry and Chemical Engineering

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

312 Downloads (Pure)

Abstract

Highly functionalised ruthenium(II) tris-bipyridine receptor 1 which acts as a selective sensor for equine cytochrome c (cyt c) is shown to destabilise the native protein conformation by around 25 degrees C. Receptors 2 and 3 do not exert this effect confirming the behaviour is a specific effect of molecular recognition between 1 and cyt c, whilst the absence of a destabilising effect on 60% acetylated cyt c demonstrates the behaviour of 1 to be protein specific. Molecular recognition also modifies the conformational properties of the target protein at room temperature as evidenced by ion-mobility spectrometry (IMS) and accelerated trypsin proteolysis.

Original language	English
Pages (from-to)	2206-2212
Number of pages	7
Journal	Organic and Biomolecular Chemistry
Volume	11
Issue number	13
Early online date	30 Jan 2013
DOIs	https://doi.org/10.1039/c3ob26251k
Publication status	Published - 2013

ASJC Scopus subject areas

Physical and Theoretical Chemistry
Organic Chemistry
Biochemistry

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10.1039/c3ob26251k

Protein destabilisation by ruthenium(II) tris-bipyridine based protein-surface mimetics
This journal is © The Royal Society of Chemistry 2013 This is an open access Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits use, distribution and reproduction for non-commercial purposes, provided the author and source are cited.
Final published version, 1.73 MB

R1928CHM: Metal complexes as Inhibitors of Protein-Protein interactions
Fletcher, N. (PI)
01/08/2007 → 31/03/2011
Project: Research

Cite this

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title = "Protein destabilisation by ruthenium(II) tris-bipyridine based protein-surface mimetics",

abstract = "Highly functionalised ruthenium(II) tris-bipyridine receptor 1 which acts as a selective sensor for equine cytochrome c (cyt c) is shown to destabilise the native protein conformation by around 25 degrees C. Receptors 2 and 3 do not exert this effect confirming the behaviour is a specific effect of molecular recognition between 1 and cyt c, whilst the absence of a destabilising effect on 60% acetylated cyt c demonstrates the behaviour of 1 to be protein specific. Molecular recognition also modifies the conformational properties of the target protein at room temperature as evidenced by ion-mobility spectrometry (IMS) and accelerated trypsin proteolysis.",

author = "Wilson, {Andrew J.} and Ault, {James R.} and Filby, {Maria H.} and Phillips, {Hazel I. A.} and Ashcroft, {Alison E.} and Fletcher, {Nicholas C.}",

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doi = "10.1039/c3ob26251k",

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AU - Wilson, Andrew J.

AU - Ault, James R.

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AU - Phillips, Hazel I. A.

AU - Ashcroft, Alison E.

AU - Fletcher, Nicholas C.

PY - 2013

Y1 - 2013

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AB - Highly functionalised ruthenium(II) tris-bipyridine receptor 1 which acts as a selective sensor for equine cytochrome c (cyt c) is shown to destabilise the native protein conformation by around 25 degrees C. Receptors 2 and 3 do not exert this effect confirming the behaviour is a specific effect of molecular recognition between 1 and cyt c, whilst the absence of a destabilising effect on 60% acetylated cyt c demonstrates the behaviour of 1 to be protein specific. Molecular recognition also modifies the conformational properties of the target protein at room temperature as evidenced by ion-mobility spectrometry (IMS) and accelerated trypsin proteolysis.

U2 - 10.1039/c3ob26251k

DO - 10.1039/c3ob26251k

M3 - Article

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JO - Organic and Biomolecular Chemistry

JF - Organic and Biomolecular Chemistry

IS - 13

ER -

Protein destabilisation by ruthenium(II) tris-bipyridine based protein-surface mimetics

Abstract

ASJC Scopus subject areas

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R1928CHM: Metal complexes as Inhibitors of Protein-Protein interactions

Cite this