Proteomics and in silico approaches to extend understanding of the glutathione transferase superfamily of the tropical liver fluke Fasciola gigantica

Russell M Morphew, Neil Eccleston, Toby J Wilkinson, John McGarry, Samirah Perally, Mark Prescott, Deborah Ward, Diana Williams, Steve Paterson, M Raman, G Ravikumar, M Khalid Saifullah, S M Abbas Abidi, Paul McVeigh, Aaron G Maule, Peter M Brophy, E James LaCourse

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)


Fasciolosis is an important foodborne, zoonotic disease of livestock and humans, with global annual health and economic losses estimated at several billion US$. Fasciola hepatica is the major species in temperate regions, while F. gigantica dominates in the tropics. In the absence of commercially available vaccines to control fasciolosis, increasing reports of resistance to current chemotherapeutic strategies and the spread of fasciolosis into new areas, new functional genomics approaches are being used to identify potential new drug targets and vaccine candidates. The glutathione transferase (GST) superfamily is both a candidate drug and vaccine target. This study reports the identification of a putatively novel Sigma class GST, present in a water-soluble cytosol extract from the tropical liver fluke F. gigantica. The GST was cloned and expressed as an enzymically active recombinant protein. This GST shares a greater identity with the human schistosomiasis GST vaccine currently at Phase II clinical trials than previously discovered F. gigantica GSTs, stimulating interest in its immuno-protective properties. In addition, in silico analysis of the GST superfamily of both F. gigantica and F. hepatica has revealed an additional Mu class GST, Omega class GSTs, and for the first time, a Zeta class member.

Original languageEnglish
Pages (from-to)5876-89
Number of pages14
JournalJournal of Proteome Research
Issue number12
Publication statusPublished - 07 Dec 2012


  • Amino Acid Sequence
  • Animals
  • Computational Biology
  • Cytosol
  • Electrophoresis, Gel, Two-Dimensional
  • Enzyme Assays
  • Escherichia coli
  • Fasciola
  • Gene Expression Profiling
  • Glutathione Transferase
  • Helminth Proteins
  • Molecular Sequence Data
  • Phylogeny
  • Protein Array Analysis
  • Proteome
  • Proteomics
  • Recombinant Proteins
  • Sequence Alignment
  • Sequence Analysis, Protein
  • Transformation, Genetic


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