Protocol to isolate and culture primary mouse feto-placental endothelial cells

Ionel Sandovici; Moritz Reiterer; Miguel Constância; Cristina M. Branco

doi:10.1016/j.xpro.2022.101721

Protocol to isolate and culture primary mouse feto-placental endothelial cells

Ionel Sandovici, Moritz Reiterer, Miguel Constância, Cristina M. Branco

School of Medicine, Dentistry and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

64 Downloads (Pure)

Abstract

In the mouse, feto-placental endothelial cells (FPEC) line the inner surface of the feto-placental blood vessels located within placental labyrinthine zone and play critical roles in placental development and function. Here, we present a detailed protocol for isolation and culture of primary mouse FPEC, as well as two complementary methods (immunohistochemistry staining and flow cytometry analysis) to assess their purity. These cells are suitable for downstream ex vivo studies to investigate their functional properties, both in normal and pathological contexts.

Original language	English
Article number	101721
Journal	STAR Protocols
Volume	3
Issue number	4
Early online date	24 Sept 2022
DOIs	https://doi.org/10.1016/j.xpro.2022.101721 https://doi.org/10.1016/j.xpro.2022.101721
Publication status	Published - 16 Dec 2022

Keywords

Cell biology
Cell culture
Cell isolation
Developmental biology
Flow cytometry/Mass cytometry
Microscopy
Tissue engineering

ASJC Scopus subject areas

General Neuroscience
General Biochemistry,Genetics and Molecular Biology
General Immunology and Microbiology

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Protocol to isolate and culture primary mouse feto-placental endothelial cell
Copyright 2022 the authors. This is an open access article published under a Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits distribution and reproduction for non-commercial purposes, provided the author and source are cited.
Final published version, 3.83 MBLicence: CC BY-NC-ND

Cite this

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AU - Sandovici, Ionel

AU - Reiterer, Moritz

AU - Constância, Miguel

AU - Branco, Cristina M.

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Y1 - 2022/12/16

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KW - Cell biology

KW - Cell culture

KW - Cell isolation

KW - Developmental biology

KW - Flow cytometry/Mass cytometry

KW - Microscopy

KW - Tissue engineering

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DO - 10.1016/j.xpro.2022.101721

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JO - STAR Protocols

JF - STAR Protocols

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M1 - 101721

ER -

Protocol to isolate and culture primary mouse feto-placental endothelial cells

Abstract

Keywords

ASJC Scopus subject areas

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