Pseudomonas aeruginosa genotypes acquired by children with cystic fibrosis by age 5-years

Timothy J Kidd; Kay A Ramsay; Suzanna Vidmar; John B Carlin; Scott C Bell; Claire E Wainwright; Keith Grimwood; ACFBAL Study Investigators

doi:10.1016/j.jcf.2014.12.007

Pseudomonas aeruginosa genotypes acquired by children with cystic fibrosis by age 5-years

Timothy J Kidd, Kay A Ramsay, Suzanna Vidmar, John B Carlin, Scott C Bell, Claire E Wainwright, Keith Grimwood, ACFBAL Study Investigators

School of Medicine, Dentistry and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

62 Citations (Scopus)

Abstract

Background

We describe Pseudomonas aeruginosa acquisitions in children with cystic fibrosis (CF) aged ≤5-years, eradication treatment efficacy, and genotypic relationships between upper and lower airway isolates and strains from non-CF sources.

Methods

Of 168 CF children aged ≤5-years in a bronchoalveolar lavage (BAL)-directed therapy trial, 155 had detailed microbiological results. Overall, 201/271 (74%) P. aeruginosa isolates from BAL and oropharyngeal cultures were available for genotyping, including those collected before and after eradication therapy.

Results

Eighty-two (53%) subjects acquired P. aeruginosa, of which most were unique strains. Initial eradication success rate was 90%, but 36 (44%) reacquired P. aeruginosa, with genotypic substitutions more common in BAL (12/14) than oropharyngeal (3/11) cultures. Moreover, oropharyngeal cultures did not predict BAL genotypes reliably.

Conclusions

CF children acquire environmental P. aeruginosa strains frequently. However, discordance between BAL and oropharyngeal strains raises questions over upper airway reservoirs and how to best determine eradication in non-expectorating children.

Original language	English
Pages (from-to)	361-369
Number of pages	9
Journal	Journal of Cystic Fibrosis
Volume	14
Issue number	3
Early online date	03 Jan 2015
DOIs	https://doi.org/10.1016/j.jcf.2014.12.007
Publication status	Published - May 2015

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10.1016/j.jcf.2014.12.007

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title = "Pseudomonas aeruginosa genotypes acquired by children with cystic fibrosis by age 5-years",

abstract = "BackgroundWe describe Pseudomonas aeruginosa acquisitions in children with cystic fibrosis (CF) aged ≤5-years, eradication treatment efficacy, and genotypic relationships between upper and lower airway isolates and strains from non-CF sources.Methods Of 168 CF children aged ≤5-years in a bronchoalveolar lavage (BAL)-directed therapy trial, 155 had detailed microbiological results. Overall, 201/271 (74%) P. aeruginosa isolates from BAL and oropharyngeal cultures were available for genotyping, including those collected before and after eradication therapy.ResultsEighty-two (53%) subjects acquired P. aeruginosa, of which most were unique strains. Initial eradication success rate was 90%, but 36 (44%) reacquired P. aeruginosa, with genotypic substitutions more common in BAL (12/14) than oropharyngeal (3/11) cultures. Moreover, oropharyngeal cultures did not predict BAL genotypes reliably.Conclusions CF children acquire environmental P. aeruginosa strains frequently. However, discordance between BAL and oropharyngeal strains raises questions over upper airway reservoirs and how to best determine eradication in non-expectorating children.",

author = "Kidd, {Timothy J} and Ramsay, {Kay A} and Suzanna Vidmar and Carlin, {John B} and Bell, {Scott C} and Wainwright, {Claire E} and Keith Grimwood and {ACFBAL Study Investigators}",

year = "2015",

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doi = "10.1016/j.jcf.2014.12.007",

language = "English",

volume = "14",

pages = "361--369",

journal = "Journal of Cystic Fibrosis",

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T1 - Pseudomonas aeruginosa genotypes acquired by children with cystic fibrosis by age 5-years

AU - Kidd, Timothy J

AU - Ramsay, Kay A

AU - Vidmar, Suzanna

AU - Carlin, John B

AU - Bell, Scott C

AU - Wainwright, Claire E

AU - Grimwood, Keith

AU - ACFBAL Study Investigators

PY - 2015/5

Y1 - 2015/5

N2 - BackgroundWe describe Pseudomonas aeruginosa acquisitions in children with cystic fibrosis (CF) aged ≤5-years, eradication treatment efficacy, and genotypic relationships between upper and lower airway isolates and strains from non-CF sources.Methods Of 168 CF children aged ≤5-years in a bronchoalveolar lavage (BAL)-directed therapy trial, 155 had detailed microbiological results. Overall, 201/271 (74%) P. aeruginosa isolates from BAL and oropharyngeal cultures were available for genotyping, including those collected before and after eradication therapy.ResultsEighty-two (53%) subjects acquired P. aeruginosa, of which most were unique strains. Initial eradication success rate was 90%, but 36 (44%) reacquired P. aeruginosa, with genotypic substitutions more common in BAL (12/14) than oropharyngeal (3/11) cultures. Moreover, oropharyngeal cultures did not predict BAL genotypes reliably.Conclusions CF children acquire environmental P. aeruginosa strains frequently. However, discordance between BAL and oropharyngeal strains raises questions over upper airway reservoirs and how to best determine eradication in non-expectorating children.

AB - BackgroundWe describe Pseudomonas aeruginosa acquisitions in children with cystic fibrosis (CF) aged ≤5-years, eradication treatment efficacy, and genotypic relationships between upper and lower airway isolates and strains from non-CF sources.Methods Of 168 CF children aged ≤5-years in a bronchoalveolar lavage (BAL)-directed therapy trial, 155 had detailed microbiological results. Overall, 201/271 (74%) P. aeruginosa isolates from BAL and oropharyngeal cultures were available for genotyping, including those collected before and after eradication therapy.ResultsEighty-two (53%) subjects acquired P. aeruginosa, of which most were unique strains. Initial eradication success rate was 90%, but 36 (44%) reacquired P. aeruginosa, with genotypic substitutions more common in BAL (12/14) than oropharyngeal (3/11) cultures. Moreover, oropharyngeal cultures did not predict BAL genotypes reliably.Conclusions CF children acquire environmental P. aeruginosa strains frequently. However, discordance between BAL and oropharyngeal strains raises questions over upper airway reservoirs and how to best determine eradication in non-expectorating children.

U2 - 10.1016/j.jcf.2014.12.007

DO - 10.1016/j.jcf.2014.12.007

M3 - Article

C2 - 25563522

SN - 1569-1993

VL - 14

SP - 361

EP - 369

JO - Journal of Cystic Fibrosis

JF - Journal of Cystic Fibrosis

IS - 3

ER -

Pseudomonas aeruginosa genotypes acquired by children with cystic fibrosis by age 5-years

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