Pseudomonas aeruginosa Isolates From a Cohort of Mexican Children With Cystic Fibrosis Show Adaptation to a Chronic Phenotype

Roberto Rosales-Reyes*, Marian Rodríguez-Alvarado, José Luis Lezana-Fernández, Joselin Yleana Sánchez-Lozano, Catalina Gayosso-Vázquez, Martha Jimena Arredondo-Mercado, Dolores Jarillo-Quijada, José Eduardo Toledano-Tableros, María Dolores Alcántar-Curiel, Nilton Lincopan, Jorge E. Vidal, Ricardo Lascurain, Miguel A. Valvano, José Ignacio Santos-Preciado

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Background: Long-term persistence of Pseudomonas aeruginosa in the lung of individuals with cystic fibrosis is associated with progressive selection of diverse genotypes and phenotypes. This bacterial adaptation leads to chronic infection, and increased morbidity and mortality. The aim of this study was to establish the prevalence, clonal relatedness, antimicrobial susceptibility and virulence-associated phenotypes of P. aeruginosa isolates in a cohort of 50 Mexican children with CF-associated chronic lung infection.
Methods: Clonal relatedness of P. aeruginosa isolates was verified by pulsed-field gel electrophoresis. The antimicrobial susceptibility was determined by an automated VITEK 2 system and/or broth micro-dilution method. Biofilm formation was quantified by the crystal violet method; swarming motility was measured on soft agar, and susceptibility to normal human serum determined by reduction of CFUs.
Results: High prevalence of P. aeruginosa colonization among Mexican children with CF was confirmed; 20% (10/49) of clones identified showed a multidrug-resistant phenotype and 8.2% (4/49) an extensive drug resistance phenotype; 26.5% (13/49) of the isolates were resistant to colistin, 42.9% (21/49) presented a phenotype of adaptation associated with chronic infection, and 79.6% (39/49) showed increased ability to survive in normal human serum.
Conclusions: This cohort of children with cystic fibrosis reveals that colonizing P. aeruginosa strains predominantly display resistance to several first-line antibiotics although most isolates were susceptible to meropenem and tobramycin; 42.9% of isolates showed a phenotype consistent with adaptation to chronic lung infection.
Original languageEnglish
JournalPediatric Infectious Disease Journal
Early online date20 May 2020
DOIs
Publication statusEarly online date - 20 May 2020

Fingerprint Dive into the research topics of 'Pseudomonas aeruginosa Isolates From a Cohort of Mexican Children With Cystic Fibrosis Show Adaptation to a Chronic Phenotype'. Together they form a unique fingerprint.

  • Cite this

    Rosales-Reyes, R., Rodríguez-Alvarado, M., Lezana-Fernández, J. L., Sánchez-Lozano, J. Y., Gayosso-Vázquez, C., Arredondo-Mercado, M. J., Jarillo-Quijada, D., Toledano-Tableros, J. E., Alcántar-Curiel, M. D., Lincopan, N., Vidal, J. E., Lascurain, R., Valvano, M. A., & Santos-Preciado, J. I. (2020). Pseudomonas aeruginosa Isolates From a Cohort of Mexican Children With Cystic Fibrosis Show Adaptation to a Chronic Phenotype. Pediatric Infectious Disease Journal. https://doi.org/10.1097/INF.0000000000002714