A two-step purification method is presented that utilizes the specific chromatographic properties of bacilli extracellular cycling ribonucleases. A double gradient system of elution is used. Initial concentration of the sample followed by reversed-phase HPLC gives high yields (90-95%) of homogeneous, active protein on both analytical and preparative scales. The procedure may be applied to the isolation of ribonucleases from different sources without significant modifications.
|Number of pages||5|
|Journal||Journal of Chromatography A|
|Publication status||Published - 03 Jun 1994|
ASJC Scopus subject areas
- Analytical Chemistry
- Organic Chemistry