Purification of bacilli ribonucleases by reversed-phase high-performance liquid chromatography

Konstantin I. Panov

doi:10.1016/0021-9673(94)80299-8

Purification of bacilli ribonucleases by reversed-phase high-performance liquid chromatography

Konstantin I. Panov^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

A two-step purification method is presented that utilizes the specific chromatographic properties of bacilli extracellular cycling ribonucleases. A double gradient system of elution is used. Initial concentration of the sample followed by reversed-phase HPLC gives high yields (90-95%) of homogeneous, active protein on both analytical and preparative scales. The procedure may be applied to the isolation of ribonucleases from different sources without significant modifications.

Original language	English
Pages (from-to)	229-233
Number of pages	5
Journal	Journal of Chromatography A
Volume	670
Issue number	1-2
DOIs	https://doi.org/10.1016/0021-9673(94)80299-8
Publication status	Published - 03 Jun 1994
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

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10.1016/0021-9673(94)80299-8

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title = "Purification of bacilli ribonucleases by reversed-phase high-performance liquid chromatography",

abstract = "A two-step purification method is presented that utilizes the specific chromatographic properties of bacilli extracellular cycling ribonucleases. A double gradient system of elution is used. Initial concentration of the sample followed by reversed-phase HPLC gives high yields (90-95%) of homogeneous, active protein on both analytical and preparative scales. The procedure may be applied to the isolation of ribonucleases from different sources without significant modifications.",

author = "Panov, {Konstantin I.}",

year = "1994",

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AU - Panov, Konstantin I.

PY - 1994/6/3

Y1 - 1994/6/3

N2 - A two-step purification method is presented that utilizes the specific chromatographic properties of bacilli extracellular cycling ribonucleases. A double gradient system of elution is used. Initial concentration of the sample followed by reversed-phase HPLC gives high yields (90-95%) of homogeneous, active protein on both analytical and preparative scales. The procedure may be applied to the isolation of ribonucleases from different sources without significant modifications.

AB - A two-step purification method is presented that utilizes the specific chromatographic properties of bacilli extracellular cycling ribonucleases. A double gradient system of elution is used. Initial concentration of the sample followed by reversed-phase HPLC gives high yields (90-95%) of homogeneous, active protein on both analytical and preparative scales. The procedure may be applied to the isolation of ribonucleases from different sources without significant modifications.

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U2 - 10.1016/0021-9673(94)80299-8

DO - 10.1016/0021-9673(94)80299-8

M3 - Article

AN - SCOPUS:0028292317

VL - 670

SP - 229

EP - 233

JO - Journal of Chromatography A

JF - Journal of Chromatography A

SN - 0021-9673

IS - 1-2

ER -

Purification of bacilli ribonucleases by reversed-phase high-performance liquid chromatography

Abstract

ASJC Scopus subject areas

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