Purification of bacilli ribonucleases by reversed-phase high-performance liquid chromatography

Konstantin I. Panov*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


A two-step purification method is presented that utilizes the specific chromatographic properties of bacilli extracellular cycling ribonucleases. A double gradient system of elution is used. Initial concentration of the sample followed by reversed-phase HPLC gives high yields (90-95%) of homogeneous, active protein on both analytical and preparative scales. The procedure may be applied to the isolation of ribonucleases from different sources without significant modifications.

Original languageEnglish
Pages (from-to)229-233
Number of pages5
JournalJournal of Chromatography A
Issue number1-2
Publication statusPublished - 03 Jun 1994
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry


Dive into the research topics of 'Purification of bacilli ribonucleases by reversed-phase high-performance liquid chromatography'. Together they form a unique fingerprint.

Cite this