Purification of measles virus with preservation of infectivity and antigenicity

W J Bellini, A Trudgett, D E McFarlin

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20 Citations (Scopus)

Abstract

Measles virus Edmonston strain was purified by ultrafiltration followed by two successive sedimentations through sucrose. Purified virus retained infectivity and, when used as an immunogen, elicited high titred antibody to measles antigens by conventional serology. The measles preparations were examined by SDS-PAGE followed by staining. In addition, following PAGE, the purity of these preparations was assessed immunochemically using antisera directed to measles and host cell antigens. The results of these studies demonstrate the utility of the purification method for the preparation of milligram quantities of relatively pure measles virus.
Original languageEnglish
Pages (from-to)633-9
Number of pages7
JournalThe Journal of general virology
Volume43
Issue number3
Publication statusPublished - Jun 1979

Keywords

  • Epitopes
  • Measles virus
  • Peptides
  • Ultrafiltration
  • Viral Proteins

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