Purification of measles virus with preservation of infectivity and antigenicity

W J Bellini; A Trudgett; D E McFarlin

Purification of measles virus with preservation of infectivity and antigenicity

W J Bellini, A Trudgett, D E McFarlin

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Measles virus Edmonston strain was purified by ultrafiltration followed by two successive sedimentations through sucrose. Purified virus retained infectivity and, when used as an immunogen, elicited high titred antibody to measles antigens by conventional serology. The measles preparations were examined by SDS-PAGE followed by staining. In addition, following PAGE, the purity of these preparations was assessed immunochemically using antisera directed to measles and host cell antigens. The results of these studies demonstrate the utility of the purification method for the preparation of milligram quantities of relatively pure measles virus.

Original language	English
Pages (from-to)	633-9
Number of pages	7
Journal	The Journal of general virology
Volume	43
Issue number	3
Publication status	Published - Jun 1979

Keywords

Epitopes
Measles virus
Peptides
Ultrafiltration
Viral Proteins

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title = "Purification of measles virus with preservation of infectivity and antigenicity",

abstract = "Measles virus Edmonston strain was purified by ultrafiltration followed by two successive sedimentations through sucrose. Purified virus retained infectivity and, when used as an immunogen, elicited high titred antibody to measles antigens by conventional serology. The measles preparations were examined by SDS-PAGE followed by staining. In addition, following PAGE, the purity of these preparations was assessed immunochemically using antisera directed to measles and host cell antigens. The results of these studies demonstrate the utility of the purification method for the preparation of milligram quantities of relatively pure measles virus.",

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T1 - Purification of measles virus with preservation of infectivity and antigenicity

AU - Bellini, W J

AU - Trudgett, A

AU - McFarlin, D E

PY - 1979/6

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N2 - Measles virus Edmonston strain was purified by ultrafiltration followed by two successive sedimentations through sucrose. Purified virus retained infectivity and, when used as an immunogen, elicited high titred antibody to measles antigens by conventional serology. The measles preparations were examined by SDS-PAGE followed by staining. In addition, following PAGE, the purity of these preparations was assessed immunochemically using antisera directed to measles and host cell antigens. The results of these studies demonstrate the utility of the purification method for the preparation of milligram quantities of relatively pure measles virus.

AB - Measles virus Edmonston strain was purified by ultrafiltration followed by two successive sedimentations through sucrose. Purified virus retained infectivity and, when used as an immunogen, elicited high titred antibody to measles antigens by conventional serology. The measles preparations were examined by SDS-PAGE followed by staining. In addition, following PAGE, the purity of these preparations was assessed immunochemically using antisera directed to measles and host cell antigens. The results of these studies demonstrate the utility of the purification method for the preparation of milligram quantities of relatively pure measles virus.

KW - Epitopes

KW - Measles virus

KW - Peptides

KW - Ultrafiltration

KW - Viral Proteins

M3 - Article

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VL - 43

SP - 633

EP - 639

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

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