Quantification of amlodipine in dried blood spot samples by high performance liquid chromatography tandem mass spectrometry

Gaoyun Chen, Feras Jirjees Sr, Abdel Al Bawab, James C. McElnay

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A sensitive and specific method, utilising high performance liquid chromatography tandem mass spectrometry (HPLC–MS/MS) was developed for the quantitative determination of amlodipine in dried blood spot (DBS) samples. Chromatographic separation was achieved using a Waters XBridge C18 column with gradient elution of a mixture of water and acetonitrile containing 0.1% formic acid (v/v). Amlodipine was quantified using a Waters Quattro Premier mass spectrometer coupled with an electro-spray ionization (ESI) source in positive ion mode. The MRM transitions of 408.9 m/z → 238.1 m/z and 408.9 → 294.0 m/zwere used to quantify and qualify amlodipine, respectively. The method was validated across the concentration range of 0.5–30 ng/mL by assessing specificity, sensitivity, linearity, precision, accuracy, recovery and matrix effect according to the Food and Drug Administration (FDA) guidelines. This method was also validated clinically within a large pharmacoepidemiological study in which amlodipine blood concentration was determined in patients who had been prescribed this medication.
Original languageEnglish
Pages (from-to)252-258
Number of pages7
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume1072
Early online date16 Nov 2017
DOIs
Publication statusPublished - 01 Jan 2018

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