Quantitation of saxitoxin in human urine using immunocapture extraction and LC-MS

William A Bragg, Alaine Garrett, Elizabeth I Hamelin, Rebecca M Coleman, Katrina Campbell, Christopher T Elliott, Rudolph C Johnson

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)
280 Downloads (Pure)

Abstract

Aim: An immunomagnetic capture protocol for use with LC-MS was developed for the quantitation of saxitoxin (STX) in human urine. Materials & methods: This method uses monoclonal antibodies coupled to magnetic beads. STX was certified reference material grade from National Research Council, Canada. Analysis was carried out using LC-MS. Results: With an extraction efficiency of 80%, accuracy and precision of 93.0-100.2% and 5.3-12.6%, respectively, and a dynamic range of 1.00-100 ng/ml, the method is well suited to quantify STX exposures based on previously reported cases. Conclusion: Compared with our previously published protocols, this method has improved selectivity, a fivefold increase in sensitivity and uses only a third of the sample volume. This method can diagnose future toxin exposures and may complement the shellfish monitoring programs worldwide.

Original languageEnglish
Pages (from-to)229-239
Number of pages11
JournalBioanalysis
Volume10
Issue number4
Early online date15 Jan 2018
DOIs
Publication statusPublished - Feb 2018

Keywords

  • antibody
  • immunocapture
  • liquid chromatography
  • magnetic beads
  • marine toxins
  • MS
  • saxitoxin

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Clinical Biochemistry
  • Medical Laboratory Technology

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