Quantitative mapping of aqueous microfluidic temperature with sub-degree resolution using fluorescence lifetime imaging microscopy

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63 Citations (Scopus)

Abstract

The use of a water-soluble, thermo-responsive polymer as a highly sensitive fluorescence-lifetime probe of microfluidic temperature is demonstrated. The fluorescence lifetime of poly(N-isopropylacrylamide) labelled with a benzofurazan fluorophore is shown to have a steep dependence on temperature around the polymer phase transition and the photophysical origin of this response is established. The use of this unusual fluorescent probe in conjunction with fluorescence lifetime imaging microscopy (FLIM) enables the spatial variation of temperature in a microfluidic device to be mapped, on the micron scale, with a resolution of less than 0.1 degrees C. This represents an increase in temperature resolution of an order of magnitude over that achieved previously by FLIM of temperature-sensitive dyes
Original languageEnglish
Pages (from-to)1267-1273
Number of pages7
JournalLab on a Chip
Volume10
Issue number10
Publication statusPublished - 2010

ASJC Scopus subject areas

  • Biochemistry
  • Bioengineering
  • Chemistry(all)
  • Biomedical Engineering

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