Radioimmunoassay quantification of adrenomedullin in gingival crevicular fluid

Fionnuala T. Lundy*, Mairead M. T. O'Hare, Bronac M. McKibben, Catherine R. Fulton, James E. Briggs, Gerard James Linden

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

Objective
To investigate whether adrenomedullin (ADM), a multifunctional peptide with key roles in host antimicrobial defence and inflammation, was present and quantifiable in human gingival crevicular fluid (GCF) and to study its relationship with periodontal health and disease.

Design
GCF samples (30 s) were collected using perio-paper strips from one diseased site in 21 subjects with periodontal disease and one healthy site from 19 control subjects with no evidence of periodontal disease. Samples were analysed by radioimmunoassay using a specific anti-human ADM antibody.

Results
Measurable adrenomedullin-like immunoreactivity (ADM-LI) was present in all the GCF samples collected. ADM-LI was significantly higher in periodontitis sites (mean 493.6 pg) than in control healthy sites (mean 248.5 pg), p = 0.0016.

Conclusion
It is concluded that ADM is present in GCF at levels at which it could have an antibacterial role in the gingival crevice and modulate the pathophysiology of periodontal inflammation.

Original languageEnglish
Pages (from-to)334-338
JournalArchives of Oral Biology
Volume51
Issue number4
Early online date12 Oct 2005
DOIs
Publication statusPublished - 01 Apr 2006

ASJC Scopus subject areas

  • Dentistry(all)

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