Raman microscopy in the diagnosis and prognosis of surgically resected nonsmall cell lung cancer

Norma Magee, James Beattie, Catherine Carland, R Davis, Kathleen McManus, Ian Bradbury, Dean Fennell, Peter Hamilton, Madeleine Ennis, John McGarvey, Joseph Elborn

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The main curative therapy for patients with nonsmall cell lung cancer is surgery. Despite this, the survival rate is only 50%, therefore it is important to more efficiently diagnose and predict prognosis for lung cancer patients. Raman spectroscopy is useful in the diagnosis of malignant and premalignant lesions. The aim of this study is to investigate the ability of Raman microscopy to diagnose lung cancer from surgically resected tissue sections, and predict the prognosis of these patients. Tumor tissue sections from curative resections are mapped by Raman microscopy and the spectra analzsed using multivariate techniques. Spectra from the tumor samples are also compared with their outcome data to define their prognostic significance. Using principal component analysis and random forest classification, Raman microscopy differentiates malignant from normal lung tissue. Principal component analysis of 34 tumor spectra predicts early postoperative cancer recurrence with a sensitivity of 73% and specificity of 74%. Spectral analysis reveals elevated porphyrin levels in the normal samples and more DNA in the tumor samples. Raman microscopy can be a useful technique for the diagnosis and prognosis of lung cancer patients receiving surgery, and for elucidating the biochemical properties of lung tumors. (C) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3323088]
Original languageEnglish
Article number026015
Pages (from-to)026015
Number of pages1
JournalJOURNAL OF BIOMEDICAL OPTICS
Volume15
Issue number2
DOIs
Publication statusPublished - 2010

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Fingerprint Dive into the research topics of 'Raman microscopy in the diagnosis and prognosis of surgically resected nonsmall cell lung cancer'. Together they form a unique fingerprint.

  • Cite this

    Magee, N., Beattie, J., Carland, C., Davis, R., McManus, K., Bradbury, I., Fennell, D., Hamilton, P., Ennis, M., McGarvey, J., & Elborn, J. (2010). Raman microscopy in the diagnosis and prognosis of surgically resected nonsmall cell lung cancer. JOURNAL OF BIOMEDICAL OPTICS, 15(2), 026015. [026015]. https://doi.org/10.1117/1.3323088