Raman spectroscopy for the detection of AGEs/ALEs

J. Renwick Beattie, John McGarvey, Alan Stitt

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Raman spectroscopy is a noninvasive, nondestructive tool for capturing multiplexed biochemical information across diverse molecular species including proteins, lipids, DNA, and mineralizations. Based on light scattering from molecules, cells, and tissues, it is possible to detect molecular fingerprints and discriminate between subtly different members of each biochemical class. Raman spectroscopy is ideal for detecting perturbations from the expected molecular structure such as those occurring during senescence and the modification of long-lived proteins by metabolic intermediates as we age. Here, we describe the sample preparation, data acquisition, signal processing, data analysis and interpretation involved in using Raman spectroscopy for detecting age-related protein modifications in complex biological tissues.
Original languageEnglish
Title of host publicationCell Senescence
EditorsLorenzo Galluzzi, Guido Kroemer
PublisherSpinger Science, Humana Press
Pages297-312
Number of pages16
Volume965
DOIs
Publication statusPublished - 2013

Publication series

NameMethods in Molecular Biology: Methods and Protocols
PublisherHumana Press

Bibliographical note

Teaching or Research: 15147
ISBN: 00000
Chapter Number: 4

ASJC Scopus subject areas

  • Medicine(all)

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