Rapid colorimetric assay for antimicrobial susceptibility testing of Pseudomonas aeruginosa

Michael Tunney, G. Ramage, T.R. Field, T.F. Moriarty, D.G. Storey

Research output: Contribution to journalArticlepeer-review

83 Citations (Scopus)

Abstract

A colorimetric assay based on the reduction of a tetrazolium salt {2,3-bis[2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT)} for rapidly determining the susceptibility of Pseudomonas aeruginosa isolates to bactericidal antibiotics is described. There was excellent agreement between the tobramycin and ofloxacin MICs determined after 5 h using the XTT assay and after 18 h using conventional methods. The data suggests that an XTT-based assay could provide a useful method for rapidly determining the susceptibility of P. aeruginosa to bactericidal antibiotics.
Original languageEnglish
Pages (from-to)1879-1881
Number of pages3
JournalAntimicrobial Agents and Chemotherapy
Volume48
Issue number5
DOIs
Publication statusPublished - May 2004

ASJC Scopus subject areas

  • Pharmacology (medical)

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