Rapid diagnostics for melioidosis: a comparative study of a novel lateral flow antigen detection assay

Gemma Robertson; Alanna Sorenson; Brenda Govan; Natkunam Ketheesan; Raymond Houghton; Hongjing Chen; David AuCoin; Michael Dillon; Robert Norton

doi:10.1099/jmm.0.000098

Rapid diagnostics for melioidosis: a comparative study of a novel lateral flow antigen detection assay

Gemma Robertson, Alanna Sorenson, Brenda Govan, Natkunam Ketheesan, Raymond Houghton, Hongjing Chen, David AuCoin, Michael Dillon, Robert Norton

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

The rapid diagnosis of septicaemic melioidosis will have an impact on reduction of mortality. Currently, this relies almost exclusively upon culture of the causative agent Burkholderia pseudomallei from clinical samples. In acute sepsis, blood is the preferred specimen for culture and therefore should be the target for a rapid diagnostic tool. A lateral flow immunoassay (LFI) for the detection of B. pseudomallei antigen has been developed. This was compared with molecular detection using the targets T3SS1 and IpxO. Forty-five clinical samples of EDTA blood, which were culture-positive, were tested using both modalities. The LFI had a sensitivity of 40 %, whilst molecular detection had a sensitivity of 20 %. The poor performance of molecular detection has been described previously and is largely related to the use of whole-blood specimens collected into blood tubes containing EDTA. Whilst suboptimal, the LFI would be an adjunct in the rapid diagnosis of melioidosis.

Original language	English
Pages (from-to)	845-8
Number of pages	4
Journal	Journal of Medical Microbiology
Volume	64
Issue number	8
DOIs	https://doi.org/10.1099/jmm.0.000098
Publication status	Published - Aug 2015
Externally published	Yes

Keywords

Antigens, Bacterial/analysis
Blood/microbiology
Burkholderia pseudomallei/immunology
Chromatography, Affinity/methods
Humans
Melioidosis/diagnosis
Molecular Diagnostic Techniques/methods
Sensitivity and Specificity
Time Factors

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10.1099/jmm.0.000098

Travel Grant
Dillon, Michael (Recipient), 18 Sep 2013
Prize: Prize (including medals and awards)

Cite this

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title = "Rapid diagnostics for melioidosis: a comparative study of a novel lateral flow antigen detection assay",

abstract = "The rapid diagnosis of septicaemic melioidosis will have an impact on reduction of mortality. Currently, this relies almost exclusively upon culture of the causative agent Burkholderia pseudomallei from clinical samples. In acute sepsis, blood is the preferred specimen for culture and therefore should be the target for a rapid diagnostic tool. A lateral flow immunoassay (LFI) for the detection of B. pseudomallei antigen has been developed. This was compared with molecular detection using the targets T3SS1 and IpxO. Forty-five clinical samples of EDTA blood, which were culture-positive, were tested using both modalities. The LFI had a sensitivity of 40 %, whilst molecular detection had a sensitivity of 20 %. The poor performance of molecular detection has been described previously and is largely related to the use of whole-blood specimens collected into blood tubes containing EDTA. Whilst suboptimal, the LFI would be an adjunct in the rapid diagnosis of melioidosis. ",

keywords = "Antigens, Bacterial/analysis, Blood/microbiology, Burkholderia pseudomallei/immunology, Chromatography, Affinity/methods, Humans, Melioidosis/diagnosis, Molecular Diagnostic Techniques/methods, Sensitivity and Specificity, Time Factors",

author = "Gemma Robertson and Alanna Sorenson and Brenda Govan and Natkunam Ketheesan and Raymond Houghton and Hongjing Chen and David AuCoin and Michael Dillon and Robert Norton",

year = "2015",

month = aug,

doi = "10.1099/jmm.0.000098",

language = "English",

volume = "64",

pages = "845--8",

journal = "Journal of Medical Microbiology",

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T2 - a comparative study of a novel lateral flow antigen detection assay

AU - Robertson, Gemma

AU - Sorenson, Alanna

AU - Govan, Brenda

AU - Ketheesan, Natkunam

AU - Houghton, Raymond

AU - Chen, Hongjing

AU - AuCoin, David

AU - Dillon, Michael

AU - Norton, Robert

PY - 2015/8

Y1 - 2015/8

N2 - The rapid diagnosis of septicaemic melioidosis will have an impact on reduction of mortality. Currently, this relies almost exclusively upon culture of the causative agent Burkholderia pseudomallei from clinical samples. In acute sepsis, blood is the preferred specimen for culture and therefore should be the target for a rapid diagnostic tool. A lateral flow immunoassay (LFI) for the detection of B. pseudomallei antigen has been developed. This was compared with molecular detection using the targets T3SS1 and IpxO. Forty-five clinical samples of EDTA blood, which were culture-positive, were tested using both modalities. The LFI had a sensitivity of 40 %, whilst molecular detection had a sensitivity of 20 %. The poor performance of molecular detection has been described previously and is largely related to the use of whole-blood specimens collected into blood tubes containing EDTA. Whilst suboptimal, the LFI would be an adjunct in the rapid diagnosis of melioidosis.

AB - The rapid diagnosis of septicaemic melioidosis will have an impact on reduction of mortality. Currently, this relies almost exclusively upon culture of the causative agent Burkholderia pseudomallei from clinical samples. In acute sepsis, blood is the preferred specimen for culture and therefore should be the target for a rapid diagnostic tool. A lateral flow immunoassay (LFI) for the detection of B. pseudomallei antigen has been developed. This was compared with molecular detection using the targets T3SS1 and IpxO. Forty-five clinical samples of EDTA blood, which were culture-positive, were tested using both modalities. The LFI had a sensitivity of 40 %, whilst molecular detection had a sensitivity of 20 %. The poor performance of molecular detection has been described previously and is largely related to the use of whole-blood specimens collected into blood tubes containing EDTA. Whilst suboptimal, the LFI would be an adjunct in the rapid diagnosis of melioidosis.

KW - Antigens, Bacterial/analysis

KW - Blood/microbiology

KW - Burkholderia pseudomallei/immunology

KW - Chromatography, Affinity/methods

KW - Humans

KW - Melioidosis/diagnosis

KW - Molecular Diagnostic Techniques/methods

KW - Sensitivity and Specificity

KW - Time Factors

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DO - 10.1099/jmm.0.000098

M3 - Article

C2 - 26055557

VL - 64

SP - 845

EP - 848

JO - Journal of Medical Microbiology

JF - Journal of Medical Microbiology

SN - 0022-2615

IS - 8

ER -

Rapid diagnostics for melioidosis: a comparative study of a novel lateral flow antigen detection assay

Abstract

Keywords

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Prizes

Travel Grant

Cite this