Real-Time Molecular Diagnosis of Tumors Using Water-Assisted Laser Desorption/Ionization Mass Spectrometry Technology

Philippe Saudemont, Jusal Quanico, Yves Marie Robin, Anna Baud, Julia Balog, Benoit Fatou, Dominique Tierny, Quentin Pascal, Kevin Minier, Mélissa Pottier, Cristian Focsa, Michael Ziskind, Zoltan Takats*, Michel Salzet, Isabelle Fournier

*Corresponding author for this work

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Histopathological diagnosis of biopsy samples and margin assessment of surgical specimens are challenging aspects in sarcoma. Using dog patient tissues, we assessed the performance of a recently developed technology for fast ex vivo molecular lipid-based diagnosis of sarcomas. The instrument is based on mass spectrometry (MS) molecular analysis through a laser microprobe operating under ambient conditions using excitation of endogenous water molecules. Classification models based on cancer/normal/necrotic, tumor grade, and subtypes showed a minimum of 97.63% correct classification. Specific markers of normal, cancer, and necrotic regions were identified by tandem MS and validated by MS imaging. Real-time detection capabilities were demonstrated by ex vivo analysis with direct interrogation of classification models. Using dog tissue samples, Saudemont et al. evaluate the performance of a mass spectrometry-based analysis of lipids for fast ex vivo diagnosis of sarcomas. This approach may improve the clinical management of sarcomas by facilitating histopathological diagnosis of biopsy samples and surgical margin assessment.

Original languageEnglish
Pages (from-to)840-851.e4
JournalCancer Cell
Volume34
Issue number5
DOIs
Publication statusPublished - 12 Nov 2018

Keywords

  • ambient ionization mass spectrometry
  • cancer
  • diagnostic
  • laser microprobe
  • molecular signatures
  • non-invasive
  • real time
  • sarcoma
  • SpiderMass
  • surgery

ASJC Scopus subject areas

  • Oncology
  • Cell Biology
  • Cancer Research

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