Redefining dilute and shoot: The evolution of the technique and its application in the analysis of foods and biological matrices by liquid chromatography mass spectrometry

Brett Greer*, Olivier Chevallier, Brian Quinn, Luis M. Botana, Christopher T. Elliott

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

2 Citations (Scopus)
28 Downloads (Pure)

Abstract

With laboratories seeking to expand analytical capabilities and create multi-class, multi-analyte methods, there has been a shift toward generic sample clean-up techniques such as “dilute-and-shoot”. Advantages of this methodology include its simplicity, minimal analyte losses, high sample throughput and number of analyte classes included. The evolution of dilute-and-shoot has permitted its use across a variety of matrices including food and biological and in various scientific fields such as food, forensics and environmental. The versatility of the technique permits the expansion of current fields of research without the usual laborious method development. There can be issues with matrix effects and robust quantitation as analyte number increases. This review provides an overview of the technique combined with liquid chromatography mass spectrometry, highlighting its power in facilitating multi-class analysis. Coupled with increases in instrument performance there is potential to employ this methodology in expanding analytical capabilities in many areas of life science research.

Original languageEnglish
Article number116284
JournalTrAC - Trends in Analytical Chemistry
Volume141
Early online date05 Apr 2021
DOIs
Publication statusPublished - 01 Aug 2021

Bibliographical note

Funding Information:
This work was supported by Agritox, a project funded by the Interreg Atlantic Area Programme through the European Regional Development Fund [EAPA 998/2018] and by the InvestNI funded Agri-Food Competence Centre project Food Fortress [11-12-17-001].

Publisher Copyright:
© 2021 The Authors

Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.

Keywords

  • Bioanalysis
  • Dilute-and-shoot
  • Food analysis
  • Liquid chromatography
  • Mass spectrometry
  • Multi-class
  • Mycotoxin
  • Sample preparation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

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