Reduction of sample matrix effects - The analysis of benzimidazole residues in serum by immunobiosensor

L. Johnsson, Robert Baxter, Joan Crooks, Michael Brandon, Christopher Elliott

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)


Regulatory authorities, the food industry and the consumer demand reliable determination of chemical contaminants present in foods. A relatively new analytical technique that addresses this need is an immunobiosensor based on surface plasmon resonance (SPR) measurements. Although a range of tests have been developed to measure residues in milk, meat, animal bile and honey, a considerable problem has been encountered with both serum and plasma samples. The high degree of non-specific binding of some sample components can lead to loss of assay robustness, increased rates of false positives and general loss of assay sensitivity. In this paper we describe a straightforward precipitation technique to remove interfering substances from serum samples to be analysed for veterinary anthelmintics by SPR. This technique enabled development of an assay to detect a wide range of benzimidazole residues in serum samples by immunobiosensor. The limit of quantification was below 5 ng/ml and coefficients of variation were about 2%.
Original languageEnglish
Pages (from-to)209-216
Number of pages8
Issue number3
Publication statusPublished - Sep 2002

ASJC Scopus subject areas

  • Food Science
  • Chemistry (miscellaneous)
  • Health, Toxicology and Mutagenesis
  • Immunology
  • Toxicology


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