Abstract
Regulatory authorities, the food industry and the consumer demand reliable determination of chemical contaminants present in foods. A relatively new analytical technique that addresses this need is an immunobiosensor based on surface plasmon resonance (SPR) measurements. Although a range of tests have been developed to measure residues in milk, meat, animal bile and honey, a considerable problem has been encountered with both serum and plasma samples. The high degree of non-specific binding of some sample components can lead to loss of assay robustness, increased rates of false positives and general loss of assay sensitivity. In this paper we describe a straightforward precipitation technique to remove interfering substances from serum samples to be analysed for veterinary anthelmintics by SPR. This technique enabled development of an assay to detect a wide range of benzimidazole residues in serum samples by immunobiosensor. The limit of quantification was below 5 ng/ml and coefficients of variation were about 2%.
Original language | English |
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Pages (from-to) | 209-216 |
Number of pages | 8 |
Journal | FOOD AND AGRICULTURAL IMMUNOLOGY |
Volume | 14 |
Issue number | 3 |
DOIs | |
Publication status | Published - Sept 2002 |
ASJC Scopus subject areas
- Food Science
- Chemistry (miscellaneous)
- Health, Toxicology and Mutagenesis
- Immunology
- Toxicology