Regulation of cyclin D1 RNA stability by SNIP1

C.P. Bracken, S.J. Wall, B. Barre, Konstantin Panov, P.M. Ajuh, N.D. Perkins

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Cyclin D1 expression represents one of the key mitogen-regulated events during the G1 phase of the cell cycle, whereas Cyclin D1 overexpression is frequently associated with human malignancy. Here, we describe a novel mechanism regulating Cyclin D1 levels. We find that SNIP1, previously identified as a regulator of Cyclin D1 expression, does not, as previously thought, primarily function as a transcriptional coactivator for this gene. Rather, SNIP1 plays a critical role in cotranscriptional or posttranscriptional Cyclin D1 mRNA stability. Moreover, we show that the majority of nucleoplasmic SNIP1 is present within a previously undescribed complex containing SkIP, THRAP3, BCLAF1, and Pinin, all proteins with reported roles in RNA processing and transcriptional regulation. We find that this complex, which we have termed the SNIP1/SkIP–associated RNA-processing complex, is coordinately recruited to both the 3' end of the Cyclin D1 gene and Cyclin D1 RNA. Significantly, SNIP1 is required for the further recruitment of the RNA processing factor U2AF65 to both the Cyclin D1 gene and RNA. This study shows a novel mechanism regulating Cyclin D1 expression and offers new insight into the role of SNIP1 and associated proteins as regulators of proliferation and cancer.
LanguageEnglish
Pages7621-7628
Number of pages8
JournalCancer Research
Volume68
Issue number18
DOIs
Publication statusPublished - 15 Sep 2008

Fingerprint

Cyclin D1
RNA Stability
RNA
bcl-1 Genes
G1 Phase
Mitogens
Neoplasms
Cell Cycle
Proteins
Genes

Cite this

Bracken, C. P., Wall, S. J., Barre, B., Panov, K., Ajuh, P. M., & Perkins, N. D. (2008). Regulation of cyclin D1 RNA stability by SNIP1. Cancer Research, 68(18), 7621-7628. https://doi.org/10.1158/0008-5472.CAN-08-1217
Bracken, C.P. ; Wall, S.J. ; Barre, B. ; Panov, Konstantin ; Ajuh, P.M. ; Perkins, N.D. / Regulation of cyclin D1 RNA stability by SNIP1. In: Cancer Research. 2008 ; Vol. 68, No. 18. pp. 7621-7628.
@article{6817e91be85348eba6e5cd1596366a92,
title = "Regulation of cyclin D1 RNA stability by SNIP1",
abstract = "Cyclin D1 expression represents one of the key mitogen-regulated events during the G1 phase of the cell cycle, whereas Cyclin D1 overexpression is frequently associated with human malignancy. Here, we describe a novel mechanism regulating Cyclin D1 levels. We find that SNIP1, previously identified as a regulator of Cyclin D1 expression, does not, as previously thought, primarily function as a transcriptional coactivator for this gene. Rather, SNIP1 plays a critical role in cotranscriptional or posttranscriptional Cyclin D1 mRNA stability. Moreover, we show that the majority of nucleoplasmic SNIP1 is present within a previously undescribed complex containing SkIP, THRAP3, BCLAF1, and Pinin, all proteins with reported roles in RNA processing and transcriptional regulation. We find that this complex, which we have termed the SNIP1/SkIP–associated RNA-processing complex, is coordinately recruited to both the 3' end of the Cyclin D1 gene and Cyclin D1 RNA. Significantly, SNIP1 is required for the further recruitment of the RNA processing factor U2AF65 to both the Cyclin D1 gene and RNA. This study shows a novel mechanism regulating Cyclin D1 expression and offers new insight into the role of SNIP1 and associated proteins as regulators of proliferation and cancer.",
author = "C.P. Bracken and S.J. Wall and B. Barre and Konstantin Panov and P.M. Ajuh and N.D. Perkins",
year = "2008",
month = "9",
day = "15",
doi = "10.1158/0008-5472.CAN-08-1217",
language = "English",
volume = "68",
pages = "7621--7628",
journal = "Cancer Research",
issn = "0008-5472",
publisher = "American Association for Cancer Research Inc.",
number = "18",

}

Bracken, CP, Wall, SJ, Barre, B, Panov, K, Ajuh, PM & Perkins, ND 2008, 'Regulation of cyclin D1 RNA stability by SNIP1', Cancer Research, vol. 68, no. 18, pp. 7621-7628. https://doi.org/10.1158/0008-5472.CAN-08-1217

Regulation of cyclin D1 RNA stability by SNIP1. / Bracken, C.P.; Wall, S.J.; Barre, B.; Panov, Konstantin; Ajuh, P.M.; Perkins, N.D.

In: Cancer Research, Vol. 68, No. 18, 15.09.2008, p. 7621-7628.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Regulation of cyclin D1 RNA stability by SNIP1

AU - Bracken, C.P.

AU - Wall, S.J.

AU - Barre, B.

AU - Panov, Konstantin

AU - Ajuh, P.M.

AU - Perkins, N.D.

PY - 2008/9/15

Y1 - 2008/9/15

N2 - Cyclin D1 expression represents one of the key mitogen-regulated events during the G1 phase of the cell cycle, whereas Cyclin D1 overexpression is frequently associated with human malignancy. Here, we describe a novel mechanism regulating Cyclin D1 levels. We find that SNIP1, previously identified as a regulator of Cyclin D1 expression, does not, as previously thought, primarily function as a transcriptional coactivator for this gene. Rather, SNIP1 plays a critical role in cotranscriptional or posttranscriptional Cyclin D1 mRNA stability. Moreover, we show that the majority of nucleoplasmic SNIP1 is present within a previously undescribed complex containing SkIP, THRAP3, BCLAF1, and Pinin, all proteins with reported roles in RNA processing and transcriptional regulation. We find that this complex, which we have termed the SNIP1/SkIP–associated RNA-processing complex, is coordinately recruited to both the 3' end of the Cyclin D1 gene and Cyclin D1 RNA. Significantly, SNIP1 is required for the further recruitment of the RNA processing factor U2AF65 to both the Cyclin D1 gene and RNA. This study shows a novel mechanism regulating Cyclin D1 expression and offers new insight into the role of SNIP1 and associated proteins as regulators of proliferation and cancer.

AB - Cyclin D1 expression represents one of the key mitogen-regulated events during the G1 phase of the cell cycle, whereas Cyclin D1 overexpression is frequently associated with human malignancy. Here, we describe a novel mechanism regulating Cyclin D1 levels. We find that SNIP1, previously identified as a regulator of Cyclin D1 expression, does not, as previously thought, primarily function as a transcriptional coactivator for this gene. Rather, SNIP1 plays a critical role in cotranscriptional or posttranscriptional Cyclin D1 mRNA stability. Moreover, we show that the majority of nucleoplasmic SNIP1 is present within a previously undescribed complex containing SkIP, THRAP3, BCLAF1, and Pinin, all proteins with reported roles in RNA processing and transcriptional regulation. We find that this complex, which we have termed the SNIP1/SkIP–associated RNA-processing complex, is coordinately recruited to both the 3' end of the Cyclin D1 gene and Cyclin D1 RNA. Significantly, SNIP1 is required for the further recruitment of the RNA processing factor U2AF65 to both the Cyclin D1 gene and RNA. This study shows a novel mechanism regulating Cyclin D1 expression and offers new insight into the role of SNIP1 and associated proteins as regulators of proliferation and cancer.

UR - http://www.scopus.com/inward/record.url?scp=54749132279&partnerID=8YFLogxK

U2 - 10.1158/0008-5472.CAN-08-1217

DO - 10.1158/0008-5472.CAN-08-1217

M3 - Article

VL - 68

SP - 7621

EP - 7628

JO - Cancer Research

T2 - Cancer Research

JF - Cancer Research

SN - 0008-5472

IS - 18

ER -

Bracken CP, Wall SJ, Barre B, Panov K, Ajuh PM, Perkins ND. Regulation of cyclin D1 RNA stability by SNIP1. Cancer Research. 2008 Sep 15;68(18):7621-7628. https://doi.org/10.1158/0008-5472.CAN-08-1217