Regulation of the pollen-specific actin-depolymerizing factor LIADF1

EG Allwood, RG Anthony, AP Smertenko, S Reichelt, BK Drobak, JH Doonan, AG Weeds, PJ Hussey*

*Corresponding author for this work

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

Pollen tube growth is dependent on a dynamic actin cytoskeleton, suggesting that actin-regulating proteins are involved. We have examined the regulation of the lily pollen-specific actin-depolymerizing factor (ADF) LIADF1. Its actin binding and depolymerizing activity is pH sensitive, inhibited by certain phosphoinositides, but not controlled by phosphorylation. Compared with its F-actin binding properties, its low activity in depolymerization assays has been used to explain why pollen ADF decorates F-actin in pollen grains. This low activity is incompatible with a role in increasing actin dynamics necessary to promote pollen tube growth. We have identified a plant homolog of actin-interacting protein, AIP1, which enhances the depolymerization of F-actin in the presence of LIADF1 by similar to60%. Both pollen ADF and pollen AIP1 bind F-actin in pollen grains but are mainly cytoplasmic in pollen tubes. Our results suggest that together these proteins remodel actin filaments as pollen grains enter and exit dormancy.

Original languageEnglish
Pages (from-to)2915-2927
Number of pages13
JournalThe Plant Cell
Volume14
Issue number11
DOIs
Publication statusPublished - Nov 2002

Keywords

  • COFILIN PHOSPHORYLATION
  • F-ACTIN
  • PHOSPHOINOSITIDES
  • DYNAMICS
  • ARABIDOPSIS
  • SEVERING PROTEIN
  • ANGIOSPERM POLLEN
  • IN-VIVO
  • FILAMENTS
  • PROTEIN-KINASE

Cite this

Allwood, EG., Anthony, RG., Smertenko, AP., Reichelt, S., Drobak, BK., Doonan, JH., ... Hussey, PJ. (2002). Regulation of the pollen-specific actin-depolymerizing factor LIADF1. The Plant Cell, 14(11), 2915-2927. https://doi.org/10.1105/tpc.005363
Allwood, EG ; Anthony, RG ; Smertenko, AP ; Reichelt, S ; Drobak, BK ; Doonan, JH ; Weeds, AG ; Hussey, PJ. / Regulation of the pollen-specific actin-depolymerizing factor LIADF1. In: The Plant Cell. 2002 ; Vol. 14, No. 11. pp. 2915-2927.
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abstract = "Pollen tube growth is dependent on a dynamic actin cytoskeleton, suggesting that actin-regulating proteins are involved. We have examined the regulation of the lily pollen-specific actin-depolymerizing factor (ADF) LIADF1. Its actin binding and depolymerizing activity is pH sensitive, inhibited by certain phosphoinositides, but not controlled by phosphorylation. Compared with its F-actin binding properties, its low activity in depolymerization assays has been used to explain why pollen ADF decorates F-actin in pollen grains. This low activity is incompatible with a role in increasing actin dynamics necessary to promote pollen tube growth. We have identified a plant homolog of actin-interacting protein, AIP1, which enhances the depolymerization of F-actin in the presence of LIADF1 by similar to60{\%}. Both pollen ADF and pollen AIP1 bind F-actin in pollen grains but are mainly cytoplasmic in pollen tubes. Our results suggest that together these proteins remodel actin filaments as pollen grains enter and exit dormancy.",
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Allwood, EG, Anthony, RG, Smertenko, AP, Reichelt, S, Drobak, BK, Doonan, JH, Weeds, AG & Hussey, PJ 2002, 'Regulation of the pollen-specific actin-depolymerizing factor LIADF1', The Plant Cell, vol. 14, no. 11, pp. 2915-2927. https://doi.org/10.1105/tpc.005363

Regulation of the pollen-specific actin-depolymerizing factor LIADF1. / Allwood, EG; Anthony, RG; Smertenko, AP; Reichelt, S; Drobak, BK; Doonan, JH; Weeds, AG; Hussey, PJ.

In: The Plant Cell, Vol. 14, No. 11, 11.2002, p. 2915-2927.

Research output: Contribution to journalArticle

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T1 - Regulation of the pollen-specific actin-depolymerizing factor LIADF1

AU - Allwood, EG

AU - Anthony, RG

AU - Smertenko, AP

AU - Reichelt, S

AU - Drobak, BK

AU - Doonan, JH

AU - Weeds, AG

AU - Hussey, PJ

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N2 - Pollen tube growth is dependent on a dynamic actin cytoskeleton, suggesting that actin-regulating proteins are involved. We have examined the regulation of the lily pollen-specific actin-depolymerizing factor (ADF) LIADF1. Its actin binding and depolymerizing activity is pH sensitive, inhibited by certain phosphoinositides, but not controlled by phosphorylation. Compared with its F-actin binding properties, its low activity in depolymerization assays has been used to explain why pollen ADF decorates F-actin in pollen grains. This low activity is incompatible with a role in increasing actin dynamics necessary to promote pollen tube growth. We have identified a plant homolog of actin-interacting protein, AIP1, which enhances the depolymerization of F-actin in the presence of LIADF1 by similar to60%. Both pollen ADF and pollen AIP1 bind F-actin in pollen grains but are mainly cytoplasmic in pollen tubes. Our results suggest that together these proteins remodel actin filaments as pollen grains enter and exit dormancy.

AB - Pollen tube growth is dependent on a dynamic actin cytoskeleton, suggesting that actin-regulating proteins are involved. We have examined the regulation of the lily pollen-specific actin-depolymerizing factor (ADF) LIADF1. Its actin binding and depolymerizing activity is pH sensitive, inhibited by certain phosphoinositides, but not controlled by phosphorylation. Compared with its F-actin binding properties, its low activity in depolymerization assays has been used to explain why pollen ADF decorates F-actin in pollen grains. This low activity is incompatible with a role in increasing actin dynamics necessary to promote pollen tube growth. We have identified a plant homolog of actin-interacting protein, AIP1, which enhances the depolymerization of F-actin in the presence of LIADF1 by similar to60%. Both pollen ADF and pollen AIP1 bind F-actin in pollen grains but are mainly cytoplasmic in pollen tubes. Our results suggest that together these proteins remodel actin filaments as pollen grains enter and exit dormancy.

KW - COFILIN PHOSPHORYLATION

KW - F-ACTIN

KW - PHOSPHOINOSITIDES

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KW - ARABIDOPSIS

KW - SEVERING PROTEIN

KW - ANGIOSPERM POLLEN

KW - IN-VIVO

KW - FILAMENTS

KW - PROTEIN-KINASE

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DO - 10.1105/tpc.005363

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JO - The Plant Cell

JF - The Plant Cell

SN - 1040-4651

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Allwood EG, Anthony RG, Smertenko AP, Reichelt S, Drobak BK, Doonan JH et al. Regulation of the pollen-specific actin-depolymerizing factor LIADF1. The Plant Cell. 2002 Nov;14(11):2915-2927. https://doi.org/10.1105/tpc.005363