Relative quantification of the proteomeic changes associated with the mycotoxin zearalenone in the H295R steroidogenesis model

O.L. Busk, D. Ndossi, S. Verhaegen, Lisa Connolly, G. Eriksen, E. Ropstad, M. Sorlie

Research output: Contribution to journalArticle

12 Citations (Scopus)
18 Downloads (Pure)

Abstract

Zearalenone (ZEN) is a mycotoxin with endocrine disrupting effects having vast economic implications in e.g. pig farming. Structurally, ZEN resembles 17b-estradiol, and thus is able to bind to estrogen receptors (ER) in target cells. Because of this, it is also classified as a non-steroidal estrogen, a phytoestrogen, a mycoestrogen, and a growth promoter. Quantitative proteomic analysis was undertaken using stable-isotope labeling by amino acids in cell culture (SILAC) upon exposure of the steroidogenesis cell model H295R with ZEN to elucidate its effect on protein regulation. ZEN significantly regulated 21 proteins, including proteins with known endocrine disrupting effects and several oncogenes. In addition, network analysis using Ingenuity Pathway Analysis showed that ZEN affected the oxidative phosphorylation pathway and the mitochondrial dysfunction pathway, both previously reported to be involved in endocrine dysfunction.
Original languageEnglish
Pages (from-to)533-542
Number of pages10
JournalToxicon
Volume58
Issue number6-7
DOIs
Publication statusPublished - Nov 2011

ASJC Scopus subject areas

  • Toxicology

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