REMA: A computer-based mapping tool for analysis of restriction sites in multiple DNA sequences

Jan Szubert; Caroline Reiff; Andrew Thorburn; Brajesh K. Singh

doi:10.1016/j.mimet.2007.01.008

REMA: A computer-based mapping tool for analysis of restriction sites in multiple DNA sequences

Jan Szubert, Caroline Reiff, Andrew Thorburn, Brajesh K. Singh

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

REMA is an interactive web-based program which predicts endonuclease cut sites in DNA sequences. It analyses Multiple sequences simultaneously and predicts the number and size of fragments as well as provides restriction maps. The users can select single or paired combinations of all commercially available enzymes. Additionally, REMA permits prediction of multiple sequence terminal fragment sizes and suggests suitable restriction enzymes for maximally discriminatory results. REMA is an easy to use, web based program which will have a wide application in molecular biology research. Availability: REMA is written in Perl and is freely available for non-commercial use. Detailed information on installation can be obtained from Jan Szubert ([email protected]) and the web based application is accessible on the internet at the URL http://www.macaulay.ac.uk/rema. Contact: [email protected]. (C) 2007 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	411-413
Number of pages	3
Journal	Journal of Microbiological Methods
Volume	69
Issue number	2
DOIs	https://doi.org/10.1016/j.mimet.2007.01.008
Publication status	Published - May 2007

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10.1016/j.mimet.2007.01.008

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title = "REMA: A computer-based mapping tool for analysis of restriction sites in multiple DNA sequences",

abstract = "REMA is an interactive web-based program which predicts endonuclease cut sites in DNA sequences. It analyses Multiple sequences simultaneously and predicts the number and size of fragments as well as provides restriction maps. The users can select single or paired combinations of all commercially available enzymes. Additionally, REMA permits prediction of multiple sequence terminal fragment sizes and suggests suitable restriction enzymes for maximally discriminatory results. REMA is an easy to use, web based program which will have a wide application in molecular biology research. Availability: REMA is written in Perl and is freely available for non-commercial use. Detailed information on installation can be obtained from Jan Szubert ([email protected]) and the web based application is accessible on the internet at the URL http://www.macaulay.ac.uk/rema. Contact: [email protected]. (C) 2007 Elsevier B.V. All rights reserved.",

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AU - Szubert, Jan

AU - Reiff, Caroline

AU - Thorburn, Andrew

AU - Singh, Brajesh K.

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AB - REMA is an interactive web-based program which predicts endonuclease cut sites in DNA sequences. It analyses Multiple sequences simultaneously and predicts the number and size of fragments as well as provides restriction maps. The users can select single or paired combinations of all commercially available enzymes. Additionally, REMA permits prediction of multiple sequence terminal fragment sizes and suggests suitable restriction enzymes for maximally discriminatory results. REMA is an easy to use, web based program which will have a wide application in molecular biology research. Availability: REMA is written in Perl and is freely available for non-commercial use. Detailed information on installation can be obtained from Jan Szubert ([email protected]) and the web based application is accessible on the internet at the URL http://www.macaulay.ac.uk/rema. Contact: [email protected]. (C) 2007 Elsevier B.V. All rights reserved.

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REMA: A computer-based mapping tool for analysis of restriction sites in multiple DNA sequences

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