Retinal VEGF mRNA measured by SYBR Green I fluorescence: A versatile approach to quantitative PCR

David Simpson; S. Feeney; C. Boyle; Alan Stitt

Retinal VEGF mRNA measured by SYBR Green I fluorescence: A versatile approach to quantitative PCR

David Simpson, S. Feeney, C. Boyle, Alan Stitt

Research output: Contribution to journal › Article › peer-review

Abstract

PURPOSE: To determine whether continuous monitoring of SYBR Green I fluorescence provides a reliable and flexible method of quantitative RT-PCR. Our aims were (i) to test whether SYBR Green I analysis could quantify a wide range of known VEGF template concentrations, (ii) to apply this method in an experimental model, and (iii) to determine whether 20 existing primer pairs could be used to quantify their cognate mRNAs.

Original language	English
Pages (from-to)	178-183
Number of pages	6
Journal	Molecular Vision
Volume	6
Issue number	24
Publication status	Published - 2000

ASJC Scopus subject areas

Ophthalmology

Cite this

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title = "Retinal VEGF mRNA measured by SYBR Green I fluorescence: A versatile approach to quantitative PCR",

abstract = "PURPOSE: To determine whether continuous monitoring of SYBR Green I fluorescence provides a reliable and flexible method of quantitative RT-PCR. Our aims were (i) to test whether SYBR Green I analysis could quantify a wide range of known VEGF template concentrations, (ii) to apply this method in an experimental model, and (iii) to determine whether 20 existing primer pairs could be used to quantify their cognate mRNAs.",

author = "David Simpson and S. Feeney and C. Boyle and Alan Stitt",

year = "2000",

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journal = "Molecular Vision",

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AU - Simpson, David

AU - Feeney, S.

AU - Boyle, C.

AU - Stitt, Alan

PY - 2000

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N2 - PURPOSE: To determine whether continuous monitoring of SYBR Green I fluorescence provides a reliable and flexible method of quantitative RT-PCR. Our aims were (i) to test whether SYBR Green I analysis could quantify a wide range of known VEGF template concentrations, (ii) to apply this method in an experimental model, and (iii) to determine whether 20 existing primer pairs could be used to quantify their cognate mRNAs.

AB - PURPOSE: To determine whether continuous monitoring of SYBR Green I fluorescence provides a reliable and flexible method of quantitative RT-PCR. Our aims were (i) to test whether SYBR Green I analysis could quantify a wide range of known VEGF template concentrations, (ii) to apply this method in an experimental model, and (iii) to determine whether 20 existing primer pairs could be used to quantify their cognate mRNAs.

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M3 - Article

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VL - 6

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JO - Molecular Vision

JF - Molecular Vision

SN - 1090-0535

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ER -

Retinal VEGF mRNA measured by SYBR Green I fluorescence: A versatile approach to quantitative PCR

Abstract

ASJC Scopus subject areas

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