Retinal VEGF mRNA measured by SYBR Green I fluorescence: A versatile approach to quantitative PCR

David Simpson, S. Feeney, C. Boyle, Alan Stitt

Research output: Contribution to journalArticlepeer-review

293 Citations (Scopus)

Abstract

PURPOSE: To determine whether continuous monitoring of SYBR Green I fluorescence provides a reliable and flexible method of quantitative RT-PCR. Our aims were (i) to test whether SYBR Green I analysis could quantify a wide range of known VEGF template concentrations, (ii) to apply this method in an experimental model, and (iii) to determine whether 20 existing primer pairs could be used to quantify their cognate mRNAs.
Original languageEnglish
Pages (from-to)178-183
Number of pages6
JournalMolecular Vision
Volume6
Issue number24
Publication statusPublished - 2000

ASJC Scopus subject areas

  • Ophthalmology

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