Rice-arsenate interactions in hydroponics: a three-gene model for tolerance

Gareth J. Norton; Meher Nigar; Paul N. Williams; Tapash Dasgupta; Andrew A. Meharg; Adam H. Price

doi:10.1093/jxb/ern098

Rice-arsenate interactions in hydroponics: a three-gene model for tolerance

Gareth J. Norton^*, Meher Nigar, Paul N. Williams, Tapash Dasgupta, Andrew A. Meharg, Adam H. Price

^*Corresponding author for this work

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

In this study, the genetic mapping of the tolerance of root growth to 13.3 muM arsenate [As(V)] using the BalaxAzucena population is improved, and candidate genes for further study are identified. A remarkable three-gene model of tolerance is advanced, which appears to involve epistatic interaction between three major genes, two on chromosome 6 and one on chromosome 10. Any combination of two of these genes inherited from the tolerant parent leads to the plant having tolerance. Lists of potential positional candidate genes are presented. These are then refined using whole genome transcriptomics data and bioinformatics. Physiological evidence is also provided that genes related to phosphate transport are unlikely to be behind the genetic loci conferring tolerance. These results offer testable hypotheses for genes related to As(V) tolerance that might offer strategies for mitigating arsenic (As) accumulation in consumed rice.

Original language	English
Pages (from-to)	2277-84
Number of pages	8
Journal	Journal of experimental botany
Volume	59
Issue number	8
DOIs	https://doi.org/10.1093/jxb/ern098
Publication status	Published - May 2008

Keywords

arsenate tolerance
Oryza sativa
HOLCUS-LANATUS
POLYMORPHISM
MAP
GENE
candidate genes
ROOTS
ORYZA-SATIVA
PHYTOCHELATIN COMPLEXES
ARSENITE
METABOLISM
epistatic interactions

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@article{47891479f46a4f0f850c90431d23bcfc,

title = "Rice-arsenate interactions in hydroponics: a three-gene model for tolerance",

abstract = "In this study, the genetic mapping of the tolerance of root growth to 13.3 muM arsenate [As(V)] using the BalaxAzucena population is improved, and candidate genes for further study are identified. A remarkable three-gene model of tolerance is advanced, which appears to involve epistatic interaction between three major genes, two on chromosome 6 and one on chromosome 10. Any combination of two of these genes inherited from the tolerant parent leads to the plant having tolerance. Lists of potential positional candidate genes are presented. These are then refined using whole genome transcriptomics data and bioinformatics. Physiological evidence is also provided that genes related to phosphate transport are unlikely to be behind the genetic loci conferring tolerance. These results offer testable hypotheses for genes related to As(V) tolerance that might offer strategies for mitigating arsenic (As) accumulation in consumed rice.",

keywords = "arsenate tolerance, Oryza sativa, HOLCUS-LANATUS, POLYMORPHISM, MAP, GENE, candidate genes, ROOTS, ORYZA-SATIVA, PHYTOCHELATIN COMPLEXES, ARSENITE, METABOLISM, epistatic interactions",

author = "Norton, {Gareth J.} and Meher Nigar and Williams, {Paul N.} and Tapash Dasgupta and Meharg, {Andrew A.} and Price, {Adam H.}",

year = "2008",

month = may,

doi = "10.1093/jxb/ern098",

language = "English",

volume = "59",

pages = "2277--84",

journal = "Journal of experimental botany",

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T1 - Rice-arsenate interactions in hydroponics: a three-gene model for tolerance

AU - Norton, Gareth J.

AU - Nigar, Meher

AU - Williams, Paul N.

AU - Dasgupta, Tapash

AU - Meharg, Andrew A.

AU - Price, Adam H.

PY - 2008/5

Y1 - 2008/5

N2 - In this study, the genetic mapping of the tolerance of root growth to 13.3 muM arsenate [As(V)] using the BalaxAzucena population is improved, and candidate genes for further study are identified. A remarkable three-gene model of tolerance is advanced, which appears to involve epistatic interaction between three major genes, two on chromosome 6 and one on chromosome 10. Any combination of two of these genes inherited from the tolerant parent leads to the plant having tolerance. Lists of potential positional candidate genes are presented. These are then refined using whole genome transcriptomics data and bioinformatics. Physiological evidence is also provided that genes related to phosphate transport are unlikely to be behind the genetic loci conferring tolerance. These results offer testable hypotheses for genes related to As(V) tolerance that might offer strategies for mitigating arsenic (As) accumulation in consumed rice.

AB - In this study, the genetic mapping of the tolerance of root growth to 13.3 muM arsenate [As(V)] using the BalaxAzucena population is improved, and candidate genes for further study are identified. A remarkable three-gene model of tolerance is advanced, which appears to involve epistatic interaction between three major genes, two on chromosome 6 and one on chromosome 10. Any combination of two of these genes inherited from the tolerant parent leads to the plant having tolerance. Lists of potential positional candidate genes are presented. These are then refined using whole genome transcriptomics data and bioinformatics. Physiological evidence is also provided that genes related to phosphate transport are unlikely to be behind the genetic loci conferring tolerance. These results offer testable hypotheses for genes related to As(V) tolerance that might offer strategies for mitigating arsenic (As) accumulation in consumed rice.

KW - arsenate tolerance

KW - Oryza sativa

KW - HOLCUS-LANATUS

KW - POLYMORPHISM

KW - MAP

KW - GENE

KW - candidate genes

KW - ROOTS

KW - ORYZA-SATIVA

KW - PHYTOCHELATIN COMPLEXES

KW - ARSENITE

KW - METABOLISM

KW - epistatic interactions

U2 - 10.1093/jxb/ern098

DO - 10.1093/jxb/ern098

M3 - Article

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VL - 59

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EP - 2284

JO - Journal of experimental botany

JF - Journal of experimental botany

SN - 0022-0957

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