Abstract
The role of RNA-binding proteins in the regulation of epigenetic processes has received increasing attention in the past decades. In particular noncoding RNAs have been shown to play a role in chromatin loop formation, recruitment of chromatin modifiers and RNA-dependent DNA methylation. In plants, the identification of specific RNA–protein interactions is now rising, facilitated by the development of specific approaches for plant tissues. Here, we present a simple one-day RNA immunoprecipitation (RIP) protocol adapted for Arabidopsis, suited for the identification of RNAs that are associated with a protein-of-interest in planta.
| Language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc.,U.S. |
| Pages | 331-343 |
| Number of pages | 13 |
| DOIs | |
| Publication status | Published - 01 Jan 2018 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1675 |
| ISSN (Print) | 1064-3745 |
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Keywords
- lncRNA
- Quantitative PCR
- Reverse transcription
- RIP
- RNA immunoprecipitation
- RNA–protein interaction
Cite this
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RNA immunoprecipitation protocol to identify protein–RNA interactions in Arabidopsis thaliana. / Mermaz, Benoit; Liu, Fuquan; Song, Jie.
Methods in Molecular Biology. Humana Press Inc.,U.S., 2018. p. 331-343 (Methods in Molecular Biology; Vol. 1675).Research output: Chapter in Book/Report/Conference proceeding › Chapter
TY - CHAP
T1 - RNA immunoprecipitation protocol to identify protein–RNA interactions in Arabidopsis thaliana
AU - Mermaz, Benoit
AU - Liu, Fuquan
AU - Song, Jie
PY - 2018/1/1
Y1 - 2018/1/1
N2 - The role of RNA-binding proteins in the regulation of epigenetic processes has received increasing attention in the past decades. In particular noncoding RNAs have been shown to play a role in chromatin loop formation, recruitment of chromatin modifiers and RNA-dependent DNA methylation. In plants, the identification of specific RNA–protein interactions is now rising, facilitated by the development of specific approaches for plant tissues. Here, we present a simple one-day RNA immunoprecipitation (RIP) protocol adapted for Arabidopsis, suited for the identification of RNAs that are associated with a protein-of-interest in planta.
AB - The role of RNA-binding proteins in the regulation of epigenetic processes has received increasing attention in the past decades. In particular noncoding RNAs have been shown to play a role in chromatin loop formation, recruitment of chromatin modifiers and RNA-dependent DNA methylation. In plants, the identification of specific RNA–protein interactions is now rising, facilitated by the development of specific approaches for plant tissues. Here, we present a simple one-day RNA immunoprecipitation (RIP) protocol adapted for Arabidopsis, suited for the identification of RNAs that are associated with a protein-of-interest in planta.
KW - lncRNA
KW - Quantitative PCR
KW - Reverse transcription
KW - RIP
KW - RNA immunoprecipitation
KW - RNA–protein interaction
UR - http://www.scopus.com/inward/record.url?scp=85032021723&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-7318-7_19
DO - 10.1007/978-1-4939-7318-7_19
M3 - Chapter
T3 - Methods in Molecular Biology
SP - 331
EP - 343
BT - Methods in Molecular Biology
PB - Humana Press Inc.,U.S.
ER -