RNA immunoprecipitation protocol to identify protein–RNA interactions in Arabidopsis thaliana

Benoit Mermaz, Fuquan Liu, Jie Song*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

The role of RNA-binding proteins in the regulation of epigenetic processes has received increasing attention in the past decades. In particular noncoding RNAs have been shown to play a role in chromatin loop formation, recruitment of chromatin modifiers and RNA-dependent DNA methylation. In plants, the identification of specific RNA–protein interactions is now rising, facilitated by the development of specific approaches for plant tissues. Here, we present a simple one-day RNA immunoprecipitation (RIP) protocol adapted for Arabidopsis, suited for the identification of RNAs that are associated with a protein-of-interest in planta.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.,U.S.
Pages331-343
Number of pages13
DOIs
Publication statusPublished - 01 Jan 2018

Publication series

NameMethods in Molecular Biology
Volume1675
ISSN (Print)1064-3745

Keywords

  • lncRNA
  • Quantitative PCR
  • Reverse transcription
  • RIP
  • RNA immunoprecipitation
  • RNA–protein interaction

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Mermaz, B., Liu, F., & Song, J. (2018). RNA immunoprecipitation protocol to identify protein–RNA interactions in Arabidopsis thaliana. In Methods in Molecular Biology (pp. 331-343). (Methods in Molecular Biology; Vol. 1675). Humana Press Inc.,U.S.. https://doi.org/10.1007/978-1-4939-7318-7_19