RNA immunoprecipitation protocol to identify protein–RNA interactions in Arabidopsis thaliana

Benoit Mermaz, Fuquan Liu, Jie Song

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

The role of RNA-binding proteins in the regulation of epigenetic processes has received increasing attention in the past decades. In particular noncoding RNAs have been shown to play a role in chromatin loop formation, recruitment of chromatin modifiers and RNA-dependent DNA methylation. In plants, the identification of specific RNA–protein interactions is now rising, facilitated by the development of specific approaches for plant tissues. Here, we present a simple one-day RNA immunoprecipitation (RIP) protocol adapted for Arabidopsis, suited for the identification of RNAs that are associated with a protein-of-interest in planta.

LanguageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.,U.S.
Pages331-343
Number of pages13
DOIs
Publication statusPublished - 01 Jan 2018

Publication series

NameMethods in Molecular Biology
Volume1675
ISSN (Print)1064-3745

Fingerprint

Immunoprecipitation
Arabidopsis
RNA
Chromatin
Proteins
Genetic Epigenesis
Untranslated RNA
RNA-Binding Proteins
DNA Methylation

Keywords

  • lncRNA
  • Quantitative PCR
  • Reverse transcription
  • RIP
  • RNA immunoprecipitation
  • RNA–protein interaction

Cite this

Mermaz, B., Liu, F., & Song, J. (2018). RNA immunoprecipitation protocol to identify protein–RNA interactions in Arabidopsis thaliana. In Methods in Molecular Biology (pp. 331-343). (Methods in Molecular Biology; Vol. 1675). Humana Press Inc.,U.S.. https://doi.org/10.1007/978-1-4939-7318-7_19
Mermaz, Benoit ; Liu, Fuquan ; Song, Jie. / RNA immunoprecipitation protocol to identify protein–RNA interactions in Arabidopsis thaliana. Methods in Molecular Biology. Humana Press Inc.,U.S., 2018. pp. 331-343 (Methods in Molecular Biology).
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Mermaz, B, Liu, F & Song, J 2018, RNA immunoprecipitation protocol to identify protein–RNA interactions in Arabidopsis thaliana. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1675, Humana Press Inc.,U.S., pp. 331-343. https://doi.org/10.1007/978-1-4939-7318-7_19

RNA immunoprecipitation protocol to identify protein–RNA interactions in Arabidopsis thaliana. / Mermaz, Benoit; Liu, Fuquan; Song, Jie.

Methods in Molecular Biology. Humana Press Inc.,U.S., 2018. p. 331-343 (Methods in Molecular Biology; Vol. 1675).

Research output: Chapter in Book/Report/Conference proceedingChapter

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Mermaz B, Liu F, Song J. RNA immunoprecipitation protocol to identify protein–RNA interactions in Arabidopsis thaliana. In Methods in Molecular Biology. Humana Press Inc.,U.S. 2018. p. 331-343. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7318-7_19