RNA interference in adult Ascaris suum - an opportunity for the development of a functional genomics platform that supports organism-, tissue- and cell-based biology in a nematode parasite

Ciaran J. McCoy; Neil D. Warnock; Louise E. Atkinson; Erwan Atcheson; Richard J. Martin; Alan P. Robertson; Aaron G. Maule; Nicola Marks; Angela Mousley

doi:10.1016/j.ijpara.2015.05.003

RNA interference in adult Ascaris suum - an opportunity for the development of a functional genomics platform that supports organism-, tissue- and cell-based biology in a nematode parasite

Ciaran J. McCoy, Neil D. Warnock, Louise E. Atkinson, Erwan Atcheson, Richard J. Martin, Alan P. Robertson, Aaron G. Maule, Nicola Marks, Angela Mousley^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

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Abstract

The sustainable control of animal parasitic nematodes requires the development of efficient functional genomics platforms to facilitate target validation and enhance anthelmintic discovery. Unfortunately, the utility of RNA interference (RNAi) for the validation of novel drug targets in nematode parasites remains problematic. Ascaris suum is an important veterinary parasite and a zoonotic pathogen. Here we show that adult A. suum is RNAi competent, and highlight the induction, spread and consistency of RNAi across multiple tissue types. This platform provides a new opportunity to undertake whole organism-, tissue- and cell-level gene function studies to enhance target validation processes for nematode parasites of veterinary/medical significance.

Original language	English
Pages (from-to)	673-678
Number of pages	6
Journal	International Journal for Parasitology
Volume	45
Issue number	11
Early online date	04 Jul 2015
DOIs	https://doi.org/10.1016/j.ijpara.2015.05.003
Publication status	Published - Sept 2015

Keywords

Ascaris suum
Nematode parasite
Reverse genetics
RNA interference

ASJC Scopus subject areas

Parasitology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ijpara.2015.05.003

RNA interference in adult Ascaris suum – an opportunity for the development of a functional genomics platform that supports organism-, tissue- and cell-based biology in a nematode parasite
2015 The Authors. Published by Elsevier Ltd. on behalf of Australian Society for Parasitology Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
Final published version, 642 KB

Louise Atkinson
- l.atkinsonqub.acuk
- School of Biological Sciences - Senior Lecturer
- Institute for Global Food Security
Person: Academic
Angela Mousley
- A.Mousleyqub.acuk
- School of Biological Sciences - Professor
- Institute for Global Food Security
Person: Academic

Cite this

McCoy, C. J., Warnock, N. D., Atkinson, L. E., Atcheson, E., Martin, R. J., Robertson, A. P., Maule, A. G., Marks, N., & Mousley, A. (2015). RNA interference in adult Ascaris suum - an opportunity for the development of a functional genomics platform that supports organism-, tissue- and cell-based biology in a nematode parasite. International Journal for Parasitology, 45(11), 673-678. https://doi.org/10.1016/j.ijpara.2015.05.003

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title = "RNA interference in adult Ascaris suum - an opportunity for the development of a functional genomics platform that supports organism-, tissue- and cell-based biology in a nematode parasite",

abstract = "The sustainable control of animal parasitic nematodes requires the development of efficient functional genomics platforms to facilitate target validation and enhance anthelmintic discovery. Unfortunately, the utility of RNA interference (RNAi) for the validation of novel drug targets in nematode parasites remains problematic. Ascaris suum is an important veterinary parasite and a zoonotic pathogen. Here we show that adult A. suum is RNAi competent, and highlight the induction, spread and consistency of RNAi across multiple tissue types. This platform provides a new opportunity to undertake whole organism-, tissue- and cell-level gene function studies to enhance target validation processes for nematode parasites of veterinary/medical significance.",

keywords = "Ascaris suum, Nematode parasite, Reverse genetics, RNA interference",

author = "McCoy, {Ciaran J.} and Warnock, {Neil D.} and Atkinson, {Louise E.} and Erwan Atcheson and Martin, {Richard J.} and Robertson, {Alan P.} and Maule, {Aaron G.} and Nicola Marks and Angela Mousley",

year = "2015",

month = sep,

doi = "10.1016/j.ijpara.2015.05.003",

language = "English",

volume = "45",

pages = "673--678",

journal = "International Journal for Parasitology",

issn = "0020-7519",

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McCoy, CJ, Warnock, ND, Atkinson, LE, Atcheson, E, Martin, RJ, Robertson, AP, Maule, AG , Marks, N & Mousley, A 2015, 'RNA interference in adult Ascaris suum - an opportunity for the development of a functional genomics platform that supports organism-, tissue- and cell-based biology in a nematode parasite', International Journal for Parasitology, vol. 45, no. 11, pp. 673-678. https://doi.org/10.1016/j.ijpara.2015.05.003

RNA interference in adult Ascaris suum - an opportunity for the development of a functional genomics platform that supports organism-, tissue- and cell-based biology in a nematode parasite. / McCoy, Ciaran J.; Warnock, Neil D.; Atkinson, Louise E. et al.
In: International Journal for Parasitology, Vol. 45, No. 11, 09.2015, p. 673-678.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - RNA interference in adult Ascaris suum - an opportunity for the development of a functional genomics platform that supports organism-, tissue- and cell-based biology in a nematode parasite

AU - McCoy, Ciaran J.

AU - Warnock, Neil D.

AU - Atkinson, Louise E.

AU - Atcheson, Erwan

AU - Martin, Richard J.

AU - Robertson, Alan P.

AU - Maule, Aaron G.

AU - Marks, Nicola

AU - Mousley, Angela

PY - 2015/9

Y1 - 2015/9

N2 - The sustainable control of animal parasitic nematodes requires the development of efficient functional genomics platforms to facilitate target validation and enhance anthelmintic discovery. Unfortunately, the utility of RNA interference (RNAi) for the validation of novel drug targets in nematode parasites remains problematic. Ascaris suum is an important veterinary parasite and a zoonotic pathogen. Here we show that adult A. suum is RNAi competent, and highlight the induction, spread and consistency of RNAi across multiple tissue types. This platform provides a new opportunity to undertake whole organism-, tissue- and cell-level gene function studies to enhance target validation processes for nematode parasites of veterinary/medical significance.

AB - The sustainable control of animal parasitic nematodes requires the development of efficient functional genomics platforms to facilitate target validation and enhance anthelmintic discovery. Unfortunately, the utility of RNA interference (RNAi) for the validation of novel drug targets in nematode parasites remains problematic. Ascaris suum is an important veterinary parasite and a zoonotic pathogen. Here we show that adult A. suum is RNAi competent, and highlight the induction, spread and consistency of RNAi across multiple tissue types. This platform provides a new opportunity to undertake whole organism-, tissue- and cell-level gene function studies to enhance target validation processes for nematode parasites of veterinary/medical significance.

KW - Ascaris suum

KW - Nematode parasite

KW - Reverse genetics

KW - RNA interference

U2 - 10.1016/j.ijpara.2015.05.003

DO - 10.1016/j.ijpara.2015.05.003

M3 - Article

SN - 0020-7519

VL - 45

SP - 673

EP - 678

JO - International Journal for Parasitology

JF - International Journal for Parasitology

IS - 11

ER -

McCoy CJ, Warnock ND, Atkinson LE, Atcheson E, Martin RJ, Robertson AP et al. RNA interference in adult Ascaris suum - an opportunity for the development of a functional genomics platform that supports organism-, tissue- and cell-based biology in a nematode parasite. International Journal for Parasitology. 2015 Sept;45(11):673-678. Epub 2015 Jul 4. doi: 10.1016/j.ijpara.2015.05.003

RNA interference in adult Ascaris suum - an opportunity for the development of a functional genomics platform that supports organism-, tissue- and cell-based biology in a nematode parasite

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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Louise Atkinson

Angela Mousley

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