RNA interference in adult Ascaris suum - an opportunity for the development of a functional genomics platform that supports organism-, tissue- and cell-based biology in a nematode parasite

Ciaran J. McCoy, Neil D. Warnock, Louise E. Atkinson, Erwan Atcheson, Richard J. Martin, Alan P. Robertson, Aaron G. Maule, Nicola Marks, Angela Mousley*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)
288 Downloads (Pure)

Abstract

The sustainable control of animal parasitic nematodes requires the development of efficient functional genomics platforms to facilitate target validation and enhance anthelmintic discovery. Unfortunately, the utility of RNA interference (RNAi) for the validation of novel drug targets in nematode parasites remains problematic. Ascaris suum is an important veterinary parasite and a zoonotic pathogen. Here we show that adult A. suum is RNAi competent, and highlight the induction, spread and consistency of RNAi across multiple tissue types. This platform provides a new opportunity to undertake whole organism-, tissue- and cell-level gene function studies to enhance target validation processes for nematode parasites of veterinary/medical significance.

Original languageEnglish
Pages (from-to)673-678
Number of pages6
JournalInternational Journal for Parasitology
Volume45
Issue number11
Early online date04 Jul 2015
DOIs
Publication statusPublished - Sep 2015

Keywords

  • Ascaris suum
  • Nematode parasite
  • Reverse genetics
  • RNA interference

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

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