Role of capsular modified heptose in the virulence of Campylobacter jejuni

Anthony Wong, Dirk Lange, Sebastien Houle, Nikolay P. Arbatsky, Miguel A. Valvano, Yuriy A. Knirel, Charles M. Dozois, Carole Creuzenet

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Abstract

The Campylobacter jejuni capsular polysaccharide is important for virulence and often contains a modified heptose. In strain ATCC 700819 (a.k.a. NCTC 11168), the modified heptose branches off from the capsular backbone and is directly exposed to the environment. We reported previously that the enzymes encoded by wcaG, mlghB and mlghC are involved in heptose modification. Here, we show that inactivation of any of these genes leads to production of capsule lacking modified heptose and alters the transcription of other capsule modification genes differentially. Inactivation of mlghB or mlghC, but not of wcaG, decreased susceptibility to bile salts and abrogated invasion of intestinal cells. All mutants showed increased sensitivity to serum killing, especially wcaG::cat, and had defects in colonization and persistence in chicken intestine, but did not show significant differences in adhesion, phagocytosis and intracellular survival in murine macrophages. Together, our findings suggest that the capsular heptose modification pathway contributes to bacterial resistance against gastrointestinal host defenses and supports bacterial persistence via its role in serum resistance and invasion of intestinal cells. Our data further suggest a dynamic regulation of expression of this pathway in the gastrointestinal tract.
Original languageEnglish
Pages (from-to)1136-1158
JournalMolecular Microbiology
Volume96
Issue number6
Early online date11 Apr 2015
DOIs
Publication statusPublished - Jun 2015

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    Wong, A., Lange, D., Houle, S., Arbatsky, N. P., Valvano, M. A., Knirel, Y. A., Dozois, C. M., & Creuzenet, C. (2015). Role of capsular modified heptose in the virulence of Campylobacter jejuni. Molecular Microbiology, 96(6), 1136-1158. https://doi.org/10.1111/mmi.12995