Routine molecular subgrouping of medulloblastoma: Bridging the divide between research and the clinic using low-cost, mass spectrometry-based DNA methylomics

E.C.  Schwalbe; H. Gohlke; D. Hicks; Gholamreza Rafiee; A Enshaei; S. Potluri; J. Matthiesen; M. Mather; R Chaston; S. Crosier; A. J.  Smith; D. Williamson; S. Bailey; S.C. Clifford

Routine molecular subgrouping of medulloblastoma: Bridging the divide between research and the clinic using low-cost, mass spectrometry-based DNA methylomics

E.C. Schwalbe, H. Gohlke, D. Hicks, Gholamreza Rafiee, A Enshaei, S. Potluri, J. Matthiesen, M. Mather, R Chaston, S. Crosier, A. J. Smith, D. Williamson, S. Bailey, S.C. Clifford

School of Electronics, Electrical Engineering and Computer Science

Research output: Other contribution

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Abstract

DNA-methylation patterns allow the subclassification of medulloblastoma, the most common childhood malignant brain tumour, into four molecular subgroups (WNT, SHH, MBGrp3 and MBGrp4). These subgroups have distinct molecular and clinico-pathological features, and their distinction is now informing future treatments and risk-stratification. Whilst microarrays to assign subgroup are suitable for research purposes, they are limited by expense, platform-specificity, sample quality requirements and practicality. Here, we aimed to develop a low-cost, array-independent, robust subgrouping assay suitable for routine quality-controlled subclassification, including scant and poor-quality samples.

Original language	English
Type	National Cancer Research Institute (NCRI)
Media of output	Abstract
Publisher	National Cancer Research Institute (NCRI)
Publication status	Published - 2014

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Schwalbe, E. C., Gohlke, H., Hicks, D., Rafiee, G., Enshaei, A., Potluri, S., Matthiesen, J., Mather, M., Chaston, R., Crosier, S., Smith, A. J., Williamson, D., Bailey, S., & Clifford, S. C. (2014). Routine molecular subgrouping of medulloblastoma: Bridging the divide between research and the clinic using low-cost, mass spectrometry-based DNA methylomics. National Cancer Research Institute (NCRI).

Schwalbe, E.C. ; Gohlke, H. ; Hicks, D. ; Rafiee, Gholamreza ; Enshaei, A ; Potluri, S. ; Matthiesen, J. ; Mather, M. ; Chaston, R ; Crosier, S. ; Smith, A. J. ; Williamson, D. ; Bailey, S. ; Clifford, S.C. / Routine molecular subgrouping of medulloblastoma: Bridging the divide between research and the clinic using low-cost, mass spectrometry-based DNA methylomics. 2014. National Cancer Research Institute (NCRI).

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title = "Routine molecular subgrouping of medulloblastoma: Bridging the divide between research and the clinic using low-cost, mass spectrometry-based DNA methylomics",

abstract = "DNA-methylation patterns allow the subclassification of medulloblastoma, the most common childhood malignant brain tumour, into four molecular subgroups (WNT, SHH, MBGrp3 and MBGrp4). These subgroups have distinct molecular and clinico-pathological features, and their distinction is now informing future treatments and risk-stratification. Whilst microarrays to assign subgroup are suitable for research purposes, they are limited by expense, platform-specificity, sample quality requirements and practicality. Here, we aimed to develop a low-cost, array-independent, robust subgrouping assay suitable for routine quality-controlled subclassification, including scant and poor-quality samples. ",

author = "E.C. Schwalbe and H. Gohlke and D. Hicks and Gholamreza Rafiee and A Enshaei and S. Potluri and J. Matthiesen and M. Mather and R Chaston and S. Crosier and Smith, {A. J.} and D. Williamson and S. Bailey and S.C. Clifford",

year = "2014",

language = "English",

publisher = "National Cancer Research Institute (NCRI)",

type = "Other",

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Schwalbe, EC, Gohlke, H, Hicks, D, Rafiee, G, Enshaei, A, Potluri, S, Matthiesen, J, Mather, M, Chaston, R, Crosier, S, Smith, AJ, Williamson, D, Bailey, S & Clifford, SC 2014, Routine molecular subgrouping of medulloblastoma: Bridging the divide between research and the clinic using low-cost, mass spectrometry-based DNA methylomics. National Cancer Research Institute (NCRI).

Routine molecular subgrouping of medulloblastoma: Bridging the divide between research and the clinic using low-cost, mass spectrometry-based DNA methylomics. / Schwalbe, E.C. ; Gohlke, H.; Hicks, D.; Rafiee, Gholamreza; Enshaei, A; Potluri, S.; Matthiesen, J.; Mather, M.; Chaston, R; Crosier, S.; Smith, A. J. ; Williamson, D.; Bailey, S.; Clifford, S.C.

National Cancer Research Institute (NCRI). 2014, National Cancer Research Institute (NCRI).

Research output: Other contribution

TY - GEN

T1 - Routine molecular subgrouping of medulloblastoma: Bridging the divide between research and the clinic using low-cost, mass spectrometry-based DNA methylomics

AU - Schwalbe, E.C.

AU - Gohlke, H.

AU - Hicks, D.

AU - Rafiee, Gholamreza

AU - Enshaei, A

AU - Potluri, S.

AU - Matthiesen, J.

AU - Mather, M.

AU - Chaston, R

AU - Crosier, S.

AU - Smith, A. J.

AU - Williamson, D.

AU - Bailey, S.

AU - Clifford, S.C.

PY - 2014

Y1 - 2014

N2 - DNA-methylation patterns allow the subclassification of medulloblastoma, the most common childhood malignant brain tumour, into four molecular subgroups (WNT, SHH, MBGrp3 and MBGrp4). These subgroups have distinct molecular and clinico-pathological features, and their distinction is now informing future treatments and risk-stratification. Whilst microarrays to assign subgroup are suitable for research purposes, they are limited by expense, platform-specificity, sample quality requirements and practicality. Here, we aimed to develop a low-cost, array-independent, robust subgrouping assay suitable for routine quality-controlled subclassification, including scant and poor-quality samples.

AB - DNA-methylation patterns allow the subclassification of medulloblastoma, the most common childhood malignant brain tumour, into four molecular subgroups (WNT, SHH, MBGrp3 and MBGrp4). These subgroups have distinct molecular and clinico-pathological features, and their distinction is now informing future treatments and risk-stratification. Whilst microarrays to assign subgroup are suitable for research purposes, they are limited by expense, platform-specificity, sample quality requirements and practicality. Here, we aimed to develop a low-cost, array-independent, robust subgrouping assay suitable for routine quality-controlled subclassification, including scant and poor-quality samples.

M3 - Other contribution

PB - National Cancer Research Institute (NCRI)

ER -