SARS-CoV-2 environmental contamination from hospitalised COVID-19 patients receiving aerosol generating procedures

RL Winslow, J Zhou, EF Windle, I Nur, R Lall, C Ji, JE Millar, P Dark, J Naisbitt, A Simonds, J Dunning, W Barclay, K Baillie, GD Perkins, Semple MG, Danny McAuley, CA Green

Research output: Contribution to journalArticlepeer-review

Abstract

Background. Continuous positive airways pressure (CPAP) and high-flow nasal oxygen (HFNO) are considered ‘aerosol-generating procedures’ (AGPs) in the treatment of COVID-19. We aimed to measure air and surface environmental contamination of SARS-CoV-2 virus when CPAP and HFNO were used, compared with supplemental oxygen, to investigate the potential risks of viral transmission to healthcare workers and patients.
Methods. 30 hospitalised patients with COVID-19 requiring supplemental oxygen, with a fraction of inspired oxygen 0.4 to maintain oxygen saturations 94%, were prospectively enrolled into an observational environmental sampling study. Participants received either supplemental oxygen, CPAP or HFNO (n=10 in each group). A nasopharyngeal swab, three air and three surface samples were collected from each participant and the clinical environment. RT qPCR analyses were performed for viral and human RNA, and positive/suspected-positive samples were cultured for the presence of biologically viable virus.
Results. Overall 21/30 (70%) of participants tested positive for SARS-CoV-2 RNA in the nasopharynx. In contrast, only 4/90 (4%) and 6/90 (7%) of all air and surface samples tested positive (positive for E and ORF1a) for viral RNA respectively, although there were an additional 10 suspected-positive samples in both air and surfaces samples (positive for E or ORF1a). CPAP/HFNO use or coughing was not associated with significantly more environmental contamination compared with supplemental oxygen use. Only one nasopharyngeal sample was culture positive.
Conclusions. The use of CPAP and HFNO to treat moderate/severe COVID-19 did not appear to be associated with substantially higher levels of air or surface viral contamination in the immediate care environment, compared with the use of supplemental oxygen.
Original languageEnglish
JournalThorax
Early online date04 Nov 2021
DOIs
Publication statusEarly online date - 04 Nov 2021

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