Savory peptides present in Moromi obtained from soy sauce fermentation of yellow soybean

H.N. Lioe, A. Apriyantono, D. Fardiaz, B. Satiawihardja, Jennifer Ames, E.L. Inns

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review


The presence of savory peptides in moromi has been investigated. Moromi was prepared by fermenting yellow soybean using Aspergillus oryzae as the starter at the first step (mold fermentation) and 20% brine solution at the next step (brine fermentation). The moromi was then ultrafiltered stepwise using membranes with MW cut-offs of 10,000, 3,000, and 500 Da, respectively. The fraction with MW <500 Da was chromatographed using Sephadex G-25 SF to yield four fractions, 1-4. Analysis of soluble peptides, NaCl content, alpha-amino nitrogen, amino acid composition, peptide profile using CE coupled with DAD, taste profile and free glutamic acid content, were performed for each fraction. Fraction 2 contained a relatively high total glutamic acid content, but a relatively low free glutamic acid content and had the highest umami taste. This fraction also had more peptides containing non-aromatic amino acids than the other fractions. The peptides present in fraction 2 may play a role, at least in part, in its intense umami taste.
Original languageEnglish
Title of host publicationChallenges in Taste Chemistry and Biology
Number of pages15
ISBN (Electronic)9780841219724
Publication statusPublished - 2004

Publication series

NameACS Symposium Series
PublisherAmerican Chemical Society
ISSN (Print)0097-6156


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