Screening and Quantitative Confirmatory Method for the Analysis of Glucocorticoids in Bovine Milk Using Liquid Chromatography-Tandem Mass Spectrometry

John Malone, Christopher Elliott, David Kennedy

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

An LC/MS/MS method was developed and validated for the simultaneous identification, confirmation, and quantification of 12 glucocorticoids in bovine milk. The method was validated in accordance with the criteria defined in Commission Decision 2002/657/EC. The developed method can detect and confirm the presence of dexamethasone, betamethasone, prednisolone, flumethasone, 6 alpha-methylprednisolone, fluorometholone, triamcinolone acetonide, prednisone, cortisone, hydrocortisone, clobetasol propionate, and clobetasol butyrate in bovine milk. Milk samples are extracted with acetonitrile; sodium chloride is subsequently added to aid partition of the milk and acetonitrile mixture. The acetonitrile extract is then subjected to liquid-liquid purification by the addition of hexane. The purified extract is evaporated to dryness and reconstituted in a water acetonitrile mixture, and determination is carried out by LC/MS/MS. The method permits analysis of up to 30 samples in 1 day.
Original languageEnglish
Pages (from-to)1656-1665
Number of pages10
JournalJournal of AOAC International
Volume93
Issue number5
Publication statusPublished - Sep 2010

ASJC Scopus subject areas

  • Analytical Chemistry
  • Agronomy and Crop Science
  • Food Science
  • Environmental Chemistry
  • Pharmacology

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