Screening for the coccidiostats halofuginone and nicarbazin in egg and chicken muscle: development of an ELISA

Anne-Catherine Huet, L. Mortier, E. Daeseleire, Terence Fodey, Christopher Elliott, P. Delahaut

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

Nicarbazin and halofuginone have been widely used as coccidiostats for the prevention and treatment of coccidiosis in poultry. It has been shown that accidental cross-contamination of feed can lead to residues of these compounds in eggs and/or muscle. This paper describes a direct competitive assay for detecting halofuginone and nicarbazin, developed as qualitative screening assay. In an optimized competitive ELISA, antibodies showed 50% binding inhibition at approximately 0.08 ng ml(-1) for halofuginone and 2.5 ng ml(-1) for dinitrocarbanilide (marker residue for nicarbazin). Extraction from the matrix was carried out with acetonitrile followed by a wash with hexane. The assay's detection capability (CCbeta) for halofuginone was
Original languageEnglish
Pages (from-to)128-134
Number of pages7
JournalFood Additives and Contaminants
Volume22
Issue number2
DOIs
Publication statusPublished - Feb 2005

ASJC Scopus subject areas

  • Food Science
  • Chemistry (miscellaneous)
  • Health, Toxicology and Mutagenesis
  • Toxicology

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