Screening for the coccidiostats halofuginone and nicarbazin in egg and chicken muscle: development of an ELISA

Anne-Catherine Huet; L. Mortier; E. Daeseleire; Terence Fodey; Christopher Elliott; P. Delahaut

doi:10.1080/02652030500038041

Screening for the coccidiostats halofuginone and nicarbazin in egg and chicken muscle: development of an ELISA

Anne-Catherine Huet, L. Mortier, E. Daeseleire, Terence Fodey, Christopher Elliott, P. Delahaut

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Nicarbazin and halofuginone have been widely used as coccidiostats for the prevention and treatment of coccidiosis in poultry. It has been shown that accidental cross-contamination of feed can lead to residues of these compounds in eggs and/or muscle. This paper describes a direct competitive assay for detecting halofuginone and nicarbazin, developed as qualitative screening assay. In an optimized competitive ELISA, antibodies showed 50% binding inhibition at approximately 0.08 ng ml(-1) for halofuginone and 2.5 ng ml(-1) for dinitrocarbanilide (marker residue for nicarbazin). Extraction from the matrix was carried out with acetonitrile followed by a wash with hexane. The assay's detection capability (CCbeta) for halofuginone was

Original language	English
Pages (from-to)	128-134
Number of pages	7
Journal	Food Additives and Contaminants
Volume	22
Issue number	2
DOIs	https://doi.org/10.1080/02652030500038041
Publication status	Published - Feb 2005

ASJC Scopus subject areas

Food Science
Chemistry (miscellaneous)
Health, Toxicology and Mutagenesis
Toxicology

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10.1080/02652030500038041

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abstract = "Nicarbazin and halofuginone have been widely used as coccidiostats for the prevention and treatment of coccidiosis in poultry. It has been shown that accidental cross-contamination of feed can lead to residues of these compounds in eggs and/or muscle. This paper describes a direct competitive assay for detecting halofuginone and nicarbazin, developed as qualitative screening assay. In an optimized competitive ELISA, antibodies showed 50% binding inhibition at approximately 0.08 ng ml(-1) for halofuginone and 2.5 ng ml(-1) for dinitrocarbanilide (marker residue for nicarbazin). Extraction from the matrix was carried out with acetonitrile followed by a wash with hexane. The assay's detection capability (CCbeta) for halofuginone was",

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AU - Huet, Anne-Catherine

AU - Mortier, L.

AU - Daeseleire, E.

AU - Fodey, Terence

AU - Elliott, Christopher

AU - Delahaut, P.

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AB - Nicarbazin and halofuginone have been widely used as coccidiostats for the prevention and treatment of coccidiosis in poultry. It has been shown that accidental cross-contamination of feed can lead to residues of these compounds in eggs and/or muscle. This paper describes a direct competitive assay for detecting halofuginone and nicarbazin, developed as qualitative screening assay. In an optimized competitive ELISA, antibodies showed 50% binding inhibition at approximately 0.08 ng ml(-1) for halofuginone and 2.5 ng ml(-1) for dinitrocarbanilide (marker residue for nicarbazin). Extraction from the matrix was carried out with acetonitrile followed by a wash with hexane. The assay's detection capability (CCbeta) for halofuginone was

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