Abstract
Nicarbazin and halofuginone have been widely used as coccidiostats for the prevention and treatment of coccidiosis in poultry. It has been shown that accidental cross-contamination of feed can lead to residues of these compounds in eggs and/or muscle. This paper describes a direct competitive assay for detecting halofuginone and nicarbazin, developed as qualitative screening assay. In an optimized competitive ELISA, antibodies showed 50% binding inhibition at approximately 0.08 ng ml(-1) for halofuginone and 2.5 ng ml(-1) for dinitrocarbanilide (marker residue for nicarbazin). Extraction from the matrix was carried out with acetonitrile followed by a wash with hexane. The assay's detection capability (CCbeta) for halofuginone was
Original language | English |
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Pages (from-to) | 128-134 |
Number of pages | 7 |
Journal | Food Additives and Contaminants |
Volume | 22 |
Issue number | 2 |
DOIs | |
Publication status | Published - Feb 2005 |
ASJC Scopus subject areas
- Food Science
- Chemistry (miscellaneous)
- Health, Toxicology and Mutagenesis
- Toxicology