Abstract
| Original language | English |
|---|---|
| Article number | 6071 |
| Number of pages | 9 |
| Journal | Scientific Reports |
| Volume | 9 |
| DOIs | |
| Publication status | Published - 15 Apr 2019 |
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Sea snake cathelicidin (Hc-cath) exerts a protective effect in mouse models of lung inflammation and infection. / Carlile, Simon R.; Shiels, Jenna; Kerrigan, Lauren; Delaney, Rebecca; Megaw, Julianne; Gilmore, Brendan F.; Weldon, Sinead; Dalton, John P.; Taggart, Clifford C.
In: Scientific Reports, Vol. 9, 6071, 15.04.2019.Research output: Contribution to journal › Article
TY - JOUR
T1 - Sea snake cathelicidin (Hc-cath) exerts a protective effect in mouse models of lung inflammation and infection
AU - Carlile, Simon R.
AU - Shiels, Jenna
AU - Kerrigan, Lauren
AU - Delaney, Rebecca
AU - Megaw, Julianne
AU - Gilmore, Brendan F.
AU - Weldon, Sinead
AU - Dalton, John P.
AU - Taggart, Clifford C.
PY - 2019/4/15
Y1 - 2019/4/15
N2 - We investigated the anti-inflammatory and antibacterial activities of Hc-cath, a cathelicidin peptide derived from the venom of the sea snake, Hydrophis cyanocyntus, using in vivo models of inflammation and infection. Hc-cath function was evaluated in in vitro, in vivo in the wax moth, Galleria mellonella, and in mouse models of intraperitoneal and respiratory Pseudomonas aeruginosa infection. Hc-Cath downregulated LPS-induced pro-inflammatory responses in macrophages and significantly improved the survival of P. aeruginosa infected G. mellonella over a 5-day period. We also demonstrated, for the first time, that Hc-cath can modulate inflammation in a mouse model of LPS-induced lung inflammation by significantly reducing the release of the pro-inflammatory cytokine and neutrophil chemoattractant, KC, resulting in reduced cellular infiltration into the lungs. Moreover, Hc-cath treatment significantly reduced the bacterial load and inflammation in mouse models of P. aeruginosa intraperitoneal and respiratory infection. The effect of Hc-cath in our studies highlights the potential to develop this peptide as a candidate for therapeutic development.
AB - We investigated the anti-inflammatory and antibacterial activities of Hc-cath, a cathelicidin peptide derived from the venom of the sea snake, Hydrophis cyanocyntus, using in vivo models of inflammation and infection. Hc-cath function was evaluated in in vitro, in vivo in the wax moth, Galleria mellonella, and in mouse models of intraperitoneal and respiratory Pseudomonas aeruginosa infection. Hc-Cath downregulated LPS-induced pro-inflammatory responses in macrophages and significantly improved the survival of P. aeruginosa infected G. mellonella over a 5-day period. We also demonstrated, for the first time, that Hc-cath can modulate inflammation in a mouse model of LPS-induced lung inflammation by significantly reducing the release of the pro-inflammatory cytokine and neutrophil chemoattractant, KC, resulting in reduced cellular infiltration into the lungs. Moreover, Hc-cath treatment significantly reduced the bacterial load and inflammation in mouse models of P. aeruginosa intraperitoneal and respiratory infection. The effect of Hc-cath in our studies highlights the potential to develop this peptide as a candidate for therapeutic development.
U2 - 10.1038/s41598-019-42537-8
DO - 10.1038/s41598-019-42537-8
M3 - Article
VL - 9
JO - Nature Scientific Reports
JF - Nature Scientific Reports
SN - 2045-2322
M1 - 6071
ER -