Seed dormancy in Arabidopsis thaliana is controlled by alternative polyadenylation of DOG1

Malgorzata Cyrek, Halina Fedak, Arkadiusz Ciesielski, Yanwu Guo, Aleksandra Sliwa, Lien Brzezniak, Katarzyna Krzyczmonik, Zbigniew Pietras, Szymon Kaczanowski, Fuquan Liu, Szymon Swiezewski

Research output: Contribution to journalArticle

36 Citations (Scopus)
372 Downloads (Pure)

Abstract

DOG1 is a key regulator of seed dormancy in Arabidopsis and other plants. Interestingly, the C-terminus of DOG1 is either absent or not conserved in many plant species. Here, we show that in Arabidopsis DOG1 transcript is subject to alternative polyadenylation. In line with this, mutants in RNA 3' processing complex display weakened seed dormancy in parallel with defects in DOG1 proximal polyadenylation site selection, suggesting that the short DOG1 transcript, is functional. This is corroborated by the finding that the proximally polyadenylated short DOG1 mRNA is translated in vivo and complements the dog1 mutation. In summary, our findings indicate that the short DOG1 protein isoform produced from the proximally polyadenylated DOG1 mRNA is a key player in the establishment of seed dormancy in Arabidopsis and characterize a set of mutants in RNA 3' processing complex required for production of proximally polyadenylated functional DOG1 transcript.
Original languageEnglish
Pages (from-to)947-955
JournalPlant Physiology
Volume170
Issue number2
Early online date30 Nov 2015
DOIs
Publication statusPublished - Feb 2016

Keywords

  • alternatively polyadenylation
  • DOG1
  • Seed dormancy

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Seed dormancy in Arabidopsis thaliana is controlled by alternative polyadenylation of DOG1'. Together they form a unique fingerprint.

  • Cite this

    Cyrek, M., Fedak, H., Ciesielski, A., Guo, Y., Sliwa, A., Brzezniak, L., Krzyczmonik, K., Pietras, Z., Kaczanowski, S., Liu, F., & Swiezewski, S. (2016). Seed dormancy in Arabidopsis thaliana is controlled by alternative polyadenylation of DOG1. Plant Physiology, 170(2), 947-955. https://doi.org/10.1104/pp.15.01483