Selective block in erythropoietin-induced differentiation of growth factor-independent retrovirally-infected TF-1 cells

M J Percy, T R Lappin, O M Buckley, M F McMullin

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2 Citations (Scopus)

Abstract

The erythroleukaemic cell line TF-1, infected with either the pBabe neo retrovirus or the retrovirus bearing the human erythropoietin (hEpo) gene, developed three growth factor-independent clones. Erythropoietin (Epo), interleukin-3 (IL-3) and granulocyte-macrophage colony stimulating factor (GM-CSF) accelerated the proliferation of these clones. Autonomous growth of the clones was independent of Epo because it was not altered by Epo anti-sense oligonucleotides, nor was Epo detectable in culture supernatants. Cells from the mutant clones could not be induced by Epo to express glycophorin A and haemoglobin synthesis was markedly reduced. Haemin reversed the block in Epo-induced haemoglobin synthesis. Acquisition of growth factor-independence appears to be linked with the selective loss of differentiation capacity. These cells may provide a useful model for the study of the mechanisms involved in leukaemic transformation.
Original languageEnglish
Pages (from-to)657-66
Number of pages10
JournalLeukemia research
Volume21
Issue number7
Publication statusPublished - Jul 1997

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