Self-assembly formation of multiple DNA-tethered lipid bilayers

S.R. Tabaei, P. Jönsson, M. Brändén, F. Höök

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38 Citations (Scopus)


Inspired by natural cell–cell junctions, where membrane-residing proteins control the separation between two or more membranes without interfering with their integrity, we report a new self-assembly route for formation of multiple highly fluid tethered lipid bilayers with the inter-membrane volume geometrically confined by membrane-anchored DNA duplexes. The formation of multiple planar membrane–membrane junctions were accomplished using disk shaped bicelles, composed of a mixture of the long-chained dimyristoyl phosphatidylcholine (DMPC) and the short-chained dihexanoyl PC further stabilized with the positively charged detergent hexadecyl-trimethyl-ammonium bromide (CTAB). Quartz crystal microbalance with dissipation (QCM-D) monitoring and fluorescence microscopy and fluorescence recovery after photobleaching (FRAP) were used to monitor the formation and to characterize the integrity of the self-assembled lipid–DNA architecture.
Original languageEnglish
JournalJournal of Structural Biology
Publication statusPublished - 01 Oct 2009


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