Sequence divergence of measles virus haemagglutinin during natural evolution and adaptation to cell culture

B. K. Rima, J. A. P. Earle, K. Baczko, V. Ter Meulen, U.G. Liebert, C. Carstens, J. Carabaña, M. Caballero, M.L. Celma, R. Fernandez-Muñoz

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Abstract

Phylogenetic analysis of the sequence of the H gene of 75 measles virus (MV) strains (32 published and 43 new sequences) was carried out. The lineage groups described from comparison of the nucleotide sequences encoding the C-terminal regions of the N protein of MV were the same as those derived from the H gene sequences in almost all cases. The databases document a number of distinct genotype switches that have occurred in Madrid (Spain). Well-documented is the complete replacement of lineage group C2, the common European genotype at that time, with that of group D3 around the autumn of 1993. No further isolations of group C2 took place in Madrid after this time. The rate of mutation of the H gene sequences of MV genotype D3 circulating in Madrid from 1993 to 1996 was very low (5 x 10(-4) per annum for a given nucleotide position). This is an order of magnitude lower than the rates of mutation observed in the HN genes of human influenza A viruses. The ratio of expressed over silent mutations indicated that the divergence was not driven by immune selection in this gene. Variations in amino acid 117 of the H protein (F or L) may be related to the ability of some strains to haemagglutinate only in the presence of salt. Adaptation of MV to different primate cell types was associated with very small numbers of mutations in the H gene. The changes could not be predicted when virus previously grown in human B cell lines was adapted to monkey Vero cells. In contrast, rodent brain-adapted viruses displayed a lot of amino acid sequence variation from normal MV strains. There was no convincing evidence for recombination between MV genotypes.
Original languageEnglish
Pages (from-to)97-106
Number of pages10
JournalJournal of General Virology
Volume78
Issue number1
DOIs
Publication statusPublished - Jan 1997

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Measles virus
Hemagglutinins
Cell Culture Techniques
Genotype
Genes
Mutation Rate
Viruses
Vero Cells
Influenza A virus
Spain
Primates
Human Influenza
Genetic Recombination
Haplorhini
Sequence Analysis
Amino Acid Sequence
Rodentia
Proteins
B-Lymphocytes
Nucleotides

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Rima, B. K., Earle, J. A. P., Baczko, K., Ter Meulen, V., Liebert, U. G., Carstens, C., ... Fernandez-Muñoz, R. (1997). Sequence divergence of measles virus haemagglutinin during natural evolution and adaptation to cell culture. Journal of General Virology, 78(1), 97-106. https://doi.org/10.1099/0022-1317-78-1-97
Rima, B. K. ; Earle, J. A. P. ; Baczko, K. ; Ter Meulen, V. ; Liebert, U.G. ; Carstens, C. ; Carabaña, J. ; Caballero, M. ; Celma, M.L. ; Fernandez-Muñoz, R. / Sequence divergence of measles virus haemagglutinin during natural evolution and adaptation to cell culture. In: Journal of General Virology. 1997 ; Vol. 78, No. 1. pp. 97-106.
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Rima, BK, Earle, JAP, Baczko, K, Ter Meulen, V, Liebert, UG, Carstens, C, Carabaña, J, Caballero, M, Celma, ML & Fernandez-Muñoz, R 1997, 'Sequence divergence of measles virus haemagglutinin during natural evolution and adaptation to cell culture', Journal of General Virology, vol. 78, no. 1, pp. 97-106. https://doi.org/10.1099/0022-1317-78-1-97

Sequence divergence of measles virus haemagglutinin during natural evolution and adaptation to cell culture. / Rima, B. K.; Earle, J. A. P.; Baczko, K.; Ter Meulen, V.; Liebert, U.G.; Carstens, C.; Carabaña, J.; Caballero, M.; Celma, M.L.; Fernandez-Muñoz, R.

In: Journal of General Virology, Vol. 78, No. 1, 01.1997, p. 97-106.

Research output: Contribution to journalArticle

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T1 - Sequence divergence of measles virus haemagglutinin during natural evolution and adaptation to cell culture

AU - Rima, B. K.

AU - Earle, J. A. P.

AU - Baczko, K.

AU - Ter Meulen, V.

AU - Liebert, U.G.

AU - Carstens, C.

AU - Carabaña, J.

AU - Caballero, M.

AU - Celma, M.L.

AU - Fernandez-Muñoz, R.

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