Serological evidence for pneumovirus infections in pigs

G. M. Allan; F. McNeilly; I. W. Walker; J. A. Young; S. Fee; A. J. Douglas; B. M. Adair

doi:10.1136/vr.142.1.8

Serological evidence for pneumovirus infections in pigs

G. M. Allan^*, F. McNeilly, I. W. Walker, J. A. Young, S. Fee, A. J. Douglas, B. M. Adair

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

A serological survey was carried out on pig sera from herds in Northern Ireland to investigate the incidence of reactivity to bovine respiratory syncytial virus (BRSV) antigens. A total of 529 pig sera from 61 herds were tested and 219 (41 per cent) were found to be reactive with BRSV-infected cell cultures in an indirect immunofluorescence test. None of the BRSV-reactive sera immunostained turkey rhinotracheitis virus-infected cell cultures, indicating specificity for BRSV epitopes. The specificity of this reactivity for BRSV antigen was confirmed by double immunolabelling, using monoclonal antibodies to BRSV and two pig sera with different reactivities to BRSV antigens. A longitudinal serological investigation of two litters of pigs indicated that BRSV-serum reactivity developed between six and 11 weeks after birth. The immunofluorescent staining pattern observed with the majority (73 per cent) of the BRSV-reactive pig sera was typical of that observed with known BRSV-reactive bovine sera. The other immunoreactive pig sera stained BRSV-infected cell cultures in an atypical staining pattern. These different reactivity patterns, combined with the results of the serum neutralisation tests, suggest that more than one serotype of a porcine pneumovirus may exist.

Original language	English
Pages (from-to)	8-12
Number of pages	5
Journal	Veterinary Record
Volume	142
Issue number	1
DOIs	https://doi.org/10.1136/vr.142.1.8
Publication status	Published - 03 Jan 1998
Externally published	Yes

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Copyright 2018 Elsevier B.V., All rights reserved.

ASJC Scopus subject areas

General Veterinary

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10.1136/vr.142.1.8

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title = "Serological evidence for pneumovirus infections in pigs",

abstract = "A serological survey was carried out on pig sera from herds in Northern Ireland to investigate the incidence of reactivity to bovine respiratory syncytial virus (BRSV) antigens. A total of 529 pig sera from 61 herds were tested and 219 (41 per cent) were found to be reactive with BRSV-infected cell cultures in an indirect immunofluorescence test. None of the BRSV-reactive sera immunostained turkey rhinotracheitis virus-infected cell cultures, indicating specificity for BRSV epitopes. The specificity of this reactivity for BRSV antigen was confirmed by double immunolabelling, using monoclonal antibodies to BRSV and two pig sera with different reactivities to BRSV antigens. A longitudinal serological investigation of two litters of pigs indicated that BRSV-serum reactivity developed between six and 11 weeks after birth. The immunofluorescent staining pattern observed with the majority (73 per cent) of the BRSV-reactive pig sera was typical of that observed with known BRSV-reactive bovine sera. The other immunoreactive pig sera stained BRSV-infected cell cultures in an atypical staining pattern. These different reactivity patterns, combined with the results of the serum neutralisation tests, suggest that more than one serotype of a porcine pneumovirus may exist.",

author = "Allan, {G. M.} and F. McNeilly and Walker, {I. W.} and Young, {J. A.} and S. Fee and Douglas, {A. J.} and Adair, {B. M.}",

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AU - Douglas, A. J.

AU - Adair, B. M.

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Serological evidence for pneumovirus infections in pigs

Abstract

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ASJC Scopus subject areas

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