Serological responses to experimental Norwalk virus infection measured using a quantitative duplex time-resolved fluorescence immunoassay

Owen Kavanagh, Mary K Estes, Amanda Reeck, Ravikiran M Raju, Antone R Opekun, Mark A Gilger, David Y Graham, Robert L Atmar

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

A quantitative duplex time-resolved fluorescence assay, dissociation-enhanced lanthanide fluorescent immunoassay (DELFIA), was developed to measure Norwalk virus (NV)-specific IgA and IgG antibodies simultaneously. The duplex assay showed superior performance by detecting seroconversion following experimental NV infection at an earlier time point than a reference total immunoglobulin enzyme-linked immunosorbent assay (ELISA).
Original languageEnglish
Pages (from-to)1187-90
Number of pages4
JournalClinical and Vaccine Immunology
Volume18
Issue number7
DOIs
Publication statusPublished - Jul 2011

Keywords

  • Immunoglobulin A
  • Antibodies, Viral
  • Caliciviridae Infections
  • Serologic Tests
  • Norwalk virus
  • Humans
  • Immunoglobulin G
  • Enzyme-Linked Immunosorbent Assay
  • Fluoroimmunoassay
  • Time Factors

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