SGT, a Hsp90beta binding partner, is accumulated in the nucleus during cell apoptosis.

Hongliang Zong

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

In this study, we reported that small glutamine-rich TPR-containing protein (SGT) interacted with not only Hsp90alpha but also Hsp90beta. Confocal analysis showed that treatment of cells with Hsp90-specific inhibitor geldanamycin (GA) disrupted the interaction of SGT with Hsp90beta and this contributed to the increase of nuclear localization of SGT in HeLa cells. The increased nuclear localization of SGT was further confirmed by the Western blotting in GA-treated HeLa cells and H1299 cells. In our previous study, SGT was found to be a new pro-apoptotic factor, so we wondered whether the sub-cellular localization of SGT was related with cell apoptosis. By confocal analysis we found that the nuclear import of SGT was significantly increased in STS-induced apoptotic HeLa cells, which implied that the sub-cellular localization of SGT was closely associated with Hsp90beta and apoptosis.
Original languageEnglish
Pages (from-to)1153-1158
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume343(4)
Issue number4
DOIs
Publication statusPublished - 19 May 2006

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Glutamine
Cell Nucleus
Apoptosis
Proteins
HeLa Cells
Far-Western Blotting
Cell Nucleus Active Transport
Cells

Cite this

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abstract = "In this study, we reported that small glutamine-rich TPR-containing protein (SGT) interacted with not only Hsp90alpha but also Hsp90beta. Confocal analysis showed that treatment of cells with Hsp90-specific inhibitor geldanamycin (GA) disrupted the interaction of SGT with Hsp90beta and this contributed to the increase of nuclear localization of SGT in HeLa cells. The increased nuclear localization of SGT was further confirmed by the Western blotting in GA-treated HeLa cells and H1299 cells. In our previous study, SGT was found to be a new pro-apoptotic factor, so we wondered whether the sub-cellular localization of SGT was related with cell apoptosis. By confocal analysis we found that the nuclear import of SGT was significantly increased in STS-induced apoptotic HeLa cells, which implied that the sub-cellular localization of SGT was closely associated with Hsp90beta and apoptosis.",
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SGT, a Hsp90beta binding partner, is accumulated in the nucleus during cell apoptosis. / Zong, Hongliang.

In: Biochemical and Biophysical Research Communications, Vol. 343(4), No. 4, 19.05.2006, p. 1153-1158.

Research output: Contribution to journalArticle

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